Quantification of anisotropy and fiber orientation in human brain histological sections.
Quantification of anisotropy and fiber orientation in human brain histological sections.
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DOI：
10.3389/fnint.2013.00003
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发表时间：
2013
影响因子：
3.5
通讯作者：
Annese J
中科院分区：
文献类型：
--
作者：
Budde MD;Annese J
弥散加权成像(DWI)提供了对中枢神经系统的微观结构和组织的无与伦比的洞察。扩散张量成像(DTI)和其他模型的扩散MRI信号提取组织的微结构属性与正常和损伤的大脑。尽管这种技术和应用很普遍，但准确和大规模的验证被证明是困难的，特别是在人脑中。在这份报告中，使用从数字公共脑库获得的人脑切片，利用结构张量分析来量化各向异性和纤维取向。导出的地图以目前的DWI实验无法达到的分辨率描绘了白质纤维的复杂程度。此外，还演示了多纤维束(即交叉纤维)和体素内纤维弥散的影响。对大脑皮层和海马区的检查证实了先前对人脑的体内和体外DTI研究的特定特征。尽管受到两个维度的限制，但生成的图像以目前DWI无法达到的分辨率提供了对白质组织的独特描述。该分析方法可用于验证从DTI和扩散信号的替代模型导出的组织属性。
Diffusion weighted imaging (DWI) has provided unparalleled insight into the microscopic structure and organization of the central nervous system. Diffusion tensor imaging (DTI) and other models of the diffusion MRI signal extract microstructural properties of tissues with relevance to the normal and injured brain. Despite the prevalence of such techniques and applications, accurate and large-scale validation has proven difficult, particularly in the human brain. In this report, human brain sections obtained from a digital public brain bank were employed to quantify anisotropy and fiber orientation using structure tensor analysis. The derived maps depict the intricate complexity of white matter fibers at a resolution not attainable with current DWI experiments. Moreover, the effects of multiple fiber bundles (i.e., crossing fibers) and intravoxel fiber dispersion were demonstrated. Examination of the cortex and hippocampal regions validated-specific features of previous in vivo and ex vivo DTI studies of the human brain. Despite the limitation to two dimensions, the resulting images provide a unique depiction of white matter organization at resolutions currently unattainable with DWI. The method of analysis may be used to validate tissue properties derived from DTI and alternative models of the diffusion signal.