Saccharomyces cerevisiae commits to a programmed cell death process in response to acetic acid
Saccharomyces cerevisiae commits to a programmed cell death process in response to acetic acid
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DOI：
10.1099/00221287-147-9-2409
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发表时间：
2001-09-01
期刊：
影响因子：
2.8
通讯作者：
Côrte-Real, M
中科院分区：
文献类型：
--
作者：
Ludovico, P;Sousa, MJ;Côrte-Real, M
最近的证据表明，在酿酒酵母中发生的凋亡表型是由氧化应激诱导的。在这里，酿酒酵母在pH 3.0下暴露于20-200 mM乙酸中200分钟导致细胞死亡。120 ~ 200 mM酸诱导的酵母死亡不受环己亚胺的抑制，并伴有典型坏死的超微结构改变。相反，与20-80 mM乙酸诱导的细胞死亡相关的改变包括：(i)环己亚胺抑制沿核膜的染色质凝聚；（ii）磷脂酰丝氨酸暴露在细胞质膜表面，通过fitc -膜联蛋白V反应显示；（iii） DNA链断裂的发生，通过TUNEL试验证明。这些结果表明醋酸可以诱导酿酒酵母的程序性细胞死亡过程与凋亡表型具有共同的特征。这一观察结果提出了一种可能性，即这种细胞死亡模式在酵母中比以前认为的更普遍，并扩展到由其他应激因子诱导的细胞死亡。
Recent evidence has revealed the occurrence of an apoptotic phenotype in Saccharomyces cerevisiae that is inducible with oxidative stress. Here, exposure of S. cerevisiae to 20-200 mM acetic acid for 200 min at pH 3.0 resulted in cell death. Yeast mortality induced by 120-200 mM acid was not inhibited by cycloheximide and was accompanied by ultrastructural alterations typical of necrosis. In contrast, alterations associated with cell death induced by 20-80 mM acetic acid included: (i) cycloheximide-inhibitable chromatin condensation along the nuclear envelope; (ii) exposure of phosphatidylserine on the surface of the cytoplasmic membrane, revealed by the FITC-annexin V reaction; and (iii) the occurrence of DNA strand breaks, demonstrated by the TUNEL assay. These results show that a programmed cell death process sharing common features with an apoptotic phenotype can be induced by acetic acid in S. cerevisiae. This observation raises the possibility of this mode of cell death being more generalized in yeasts than previously considered and extended to cell death induced by other stress agents.