Spatial cytokine distribution following traumatic injury
Spatial cytokine distribution following traumatic injury
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DOI：
10.1016/j.cyto.2014.01.001
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发表时间：
2014-04-01
期刊：
影响因子：
3.8
通讯作者：
Boyd, Jonathan W.
中科院分区：
文献类型：
--
作者：
Currie, Holly N.;Loos, Matthew S.;Boyd, Jonathan W.
外伤后细胞因子浓度的时间变化已被广泛研究。关于创伤性损伤后细胞因子浓度的空间差异知之甚少。本研究的主要目的是确定细胞因子和损伤区 (ZOI) 之间的空间关系。对从遭受开放性股骨骨折的大鼠获得的肌肉和血管组织进行分析，以确定是否存在可用作 ZOI 生物标志物的空间细胞因子梯度。在骨折后的 4 个时间点从 3 个不同位置采集样本：骨折部位、距骨折 1 厘米以及对侧腿。对每个样品中 IL-6、IL-1 α、IL-1 β、IL-2、GM-CSF、TNF-α 和 MIP-1 α 的浓度进行定量。发现了细胞因子浓度的时间和空间调节变化。 IL-6 作为 ZOI 生物标志物最有前景，其空间浓度具有统计学上的差异，且与两种组织中距骨折的距离成反比。 IL-1β和IL-2在两种组织中也显示出浓度的空间差异，而GM-CSF、MIP-1a和TNF-α在血管样品中显示出空间差异。这些结果表明，创伤性损伤后存在空间细胞因子梯度，代表可用于定义 ZOI 的潜在生物标志物。 (C) 2014 Elsevier Ltd. 保留所有权利。
Temporal changes in cytokine concentrations following traumatic injuries have been extensively studied. Less is known regarding spatial differences in cytokine concentrations following traumatic injury. The primary aim of this study was to determine the spatial relationship between cytokines and the zone of injury (ZOI). Muscle and vessel tissues obtained from rats subjected to an open femoral fracture were analyzed to determine if spatial cytokine gradients exist that could potentially be used as biomarkers of the ZOI. Samples were collected at 4 time points following fracture from 3 distinct locations: at the fracture site, 1-cm away from the fracture, and from the opposite leg. The concentrations of IL-6, IL-1 alpha, IL-1 beta, IL-2, GM-CSF, TNF-alpha, and MIP-1 alpha were quantified in each sample. Temporally and spatially regulated variations in cytokine concentrations were found. IL-6 showed the most promise as a ZOI biomarker with statistically different spatial concentrations that were inversely proportional to the distance from the fracture in both tissues. IL-1 beta and IL-2 also showed spatial differences in concentration in both tissues, while GM-CSF, MIP-la, and TNF-alpha showed spatial differences in vessel samples. These results demonstrate that spatial cytokine gradients exist following traumatic injury, representing potential biomarkers that may be used to define the ZOI. (C) 2014 Elsevier Ltd. All rights reserved.