Circulating activated endothelial cells in sickle cell anemia
Circulating activated endothelial cells in sickle cell anemia
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DOI：
10.1056/nejm199711273372203
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发表时间：
1997-11-27
影响因子：
158.5
通讯作者：
Hebbel, RP
中科院分区：
文献类型：
--
作者：
Solovey, A;Lin, Y;Hebbel, RP
背景：血管壁参与了镰状细胞病的发病机制。为确定镰状细胞性贫血患者的血管内皮细胞是否被激活，我们对镰状细胞贫血患者的循环内皮细胞的数量、来源和表面表型进行了研究。方法采用免疫组织化学方法对循环内皮细胞进行计数，并用免疫荧光显微镜观察循环内皮细胞的表面表型。结果正常献血员、镰状细胞性贫血患者和非S所致溶血性贫血患者循环内皮细胞平均(+/-SD)数分别为2.6+/1.6、3.0+/-2.6和2.0+/-0.8/毫升。伴有急性疼痛发作的镰状细胞性贫血患者每毫升血液中循环内皮细胞数为22.8+/-18.2(P
Background The vascular wall participates in the pathogenesis of sickle cell disease. To determine whether the endothelium is activated in this disease, we studied the number, origin, and surface phenotype of circulating endothelial cells in patients with sickle cell anemia.Methods We used immunohistochemical examination of buffy-coat smears to enumerate circulating endothelial cells, and we evaluated the surface phenotype by applying immunofluorescence microscopy to preparations of circulating endothelial cells. A panel of antibodies was used, including a specific anti-endothelial-cell antibody, P1H12.Results Mean (+/-SD) numbers of circulating endothelial cells in normal blood donors, patients with sickle cell trait, and patients with hemolytic anemias not due to hemoglobin S were 2.6+/-1.6, 3.0+/-2.6, and 2.0+/-0.8 per milliliter of whole blood, respectively. Patients with sickle cell anemia who presented with acute painful episodes had 22.8+/-18.2 circulating endothelial cells per milliliter of blood (P