PEPTIDE TRANSLOCATION BY VARIANTS OF THE TRANSPORTER ASSOCIATED WITH ANTIGEN-PROCESSING
PEPTIDE TRANSLOCATION BY VARIANTS OF THE TRANSPORTER ASSOCIATED WITH ANTIGEN-PROCESSING
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DOI：
10.1126/science.8266106
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发表时间：
1993-12-24
期刊：
影响因子：
56.9
通讯作者：
PLOEGH, HL
中科院分区：
文献类型：
--
作者：
HEEMELS, MT;SCHUMACHER, TNM;PLOEGH, HL
主要组织相容性复合体（MHC）I类分子与通过与抗原加工相关的转运蛋白（TAP）从胞质溶胶递送至内质网腔的肽相关。SHR和刘易斯大鼠的肝微粒体表达TAP的不同等位基因（分别为cim（B）和cim（a）），积累不同的肽组。使用MHC同源大鼠分配这种差异的MHC，独立的I类产品的表达。cim（a）和cim（B）TAP复合物均使具有疏水羧基末端的肽易位，但具有羧基末端His、Lys或Arg残基的肽的易位是cim（a）所特有的。因此，TAP肽转运子的特异性限制了可用于抗原呈递的肽。
Major histocompatibility complex (MHC) class I molecules associate with peptides that are delivered from the cytosol to the lumen of the endoplasmic reticulum by the transporter associated with antigen processing (TAP). Liver microsomes of SHR and Lewis rats, which express different alleles of TAP (cim(b) and cim(a), respectively), accumulate different sets of peptides. Use of MHC congenic rats assigned this difference to the MHC, independent of the class I products expressed. Both the cim(a) and cim(b) TAP complexes translocate peptides with a hydrophobic carboxyl terminus, but translocation of peptides with a carboxyl-terminal His, Lys, or Arg residue is unique to cim(a). Thus, the specificity of the TAP peptide translocator restricts the peptides available for antigen presentation.