A new collagenase blend increases the number of islets isolated from mouse pancreas
A new collagenase blend increases the number of islets isolated from mouse pancreas
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DOI：
10.4161/isl.1.3.9556
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发表时间：
2009-11-01
期刊：
影响因子：
2.2
通讯作者：
Lammert, Eckhard
中科院分区：
文献类型：
--
作者：
Yesil, Pinar;Michel, Marcus;Lammert, Eckhard
糖尿病是工业化国家的主要代谢紊乱。由于胰岛在I型和II型糖尿病中起关键作用，因此从胰腺组织分离胰岛代表了糖尿病研究中的重要步骤。然而，迄今为止，只有一小部分位于任何特定胰腺内的所有胰岛是通过使用目前可用的酶混合物收获的。这适用于从小鼠和人胰腺分离胰岛。在本研究中，将新开发的Liberase TL Research Grade与广泛使用的Liberase RI进行比较，以研究胶原酶纯度增加对胰岛产量的影响。该研究表明，在Liberase T生产过程中减少胶原酶的降解产物，可使从小鼠胰腺中分离的胰岛数量显著增加28%，因此，预计将降低小鼠数量，从而降低糖尿病研究的成本。此外，该研究还指出了增加从人类胰腺分离的胰岛的数量和质量的可能性，因为只有有限的供体库存在。
Diabetes is a predominant metabolic disorder in the industrialized nations. Since pancreatic islets play a key role in type I and type II diabetes, the isolation of islets from pancreatic tissues represents an important step in diabetes research. However, to date, only a small fraction of all islets, resident within any given pancreas, are harvested by using currently available enzyme blends. This holds true for islet isolation from both the mouse and the human pancreas. In the present study, the newly developed Liberase TL Research Grade was compared to the widely used Liberase RI to investigate the effect of increased collagenase purity on islet yield. The study shows that reducing the degradation products of collagenases during Liberase T production significantly increases the number of islets isolated from the mouse pancreas by 28%, and, therefore, is expected to lower the numbers of mice and resulting costs for diabetes research accordingly. Furthermore, this study also points to a possibility to increase the number and mass of islets isolated from human pancreases, for which only a limited donor pool exists.