Antigen‐presenting function of human peritoneum mesothelial cells
Antigen‐presenting function of human peritoneum mesothelial cells
复制标题
人腹膜间皮细胞的抗原呈递功能
DOI：
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发表时间：
1995
影响因子：
4.6
通讯作者：
Fabrizio Manca
中科院分区：
文献类型：
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作者：
M. T. Valle;M. L. Degl’Innocenti;R. Bertelli;P. Facchetti;F. Perfumo;Daniela Fenoglio;A. Kunkl;R. Gusmano;Fabrizio Manca
在体外培养终末期肾衰竭（ESRF）患者进行连续腹膜透析的腹膜导管手术期间获得的人腹膜网膜碎片中的间皮细胞（MC）。 MC表现出与巨噬细胞不同的表型，但MHC II类分子表达良好。因此，通过用可溶性抗原（破伤风类毒素和纯化蛋白衍生物（PPD））或用微粒抗原（白色念珠菌体）脉冲来测试MC系的抗原呈递能力。使用去除贴壁单核细胞的自体外周血单核细胞 (PBMC) 和与 MHC II 类抗原 DR2 匹配的个体产生的克隆 T 细胞来测试抗原呈递功能。 MC 有效地将可溶性和粒状抗原呈递给自体和 MHC 相容的同种异体淋巴细胞，表明它们具有内吞/吞噬活性和处理/呈递能力。 MC与人重组干扰素γ（IFN-γ）预孵育上调MHC II类和细胞间粘附分子-I（ICAM-I）表达，但对抗原呈递功能的影响并不一致。由于 MC 是腹膜环境的重要组成部分，因此它们可能与巨噬细胞一起参与特定 T 细胞的激活以及针对各种病原体的局部细胞介导的免疫的产生。
Mesothelial cells (MC) from human peritoneal omentum fragments obtained during surgical insertion of peritoneal catheters for continuous peritoneal dialysis in end stage renal failure (ESRF) patients were cultured in vitro. MC exhibited a phenotype different from macrophages, but MHC class II molecules were well expressed. Therefore MC lines were tested for antigen‐presenting capacity by pulsing with soluble antigens (tetanus toxoid and purified protein derivative (PPD)) or with a corpusculate antigen (Candida albicans bodies). Autologous peripheral blood mononuclear cells (PBMC) depleted of adherent monocytes and cloned T cells generated from an individual matched for the MHC class II antigen DR2 were used to test antigen‐presenting function. MC effectively presented the soluble and corpusculate antigens to autologous and MHC‐compatible allogeneic lymphocytes, indicating that they are endowed with both endocytic/phagocytic activity and with processing/presenting capacity. Preincubation of MC with human recombinant interferon‐gamma (IFN‐γ) up‐regulated MHC class II and intercellular adhesion molecule‐I (ICAM‐I) expression, but the effect on antigen‐presenting function was not consistent. Since MC are an important component of the peritoneal environment, they may participate, along with macrophages, in activation of specific T cells and in the generation of local cell‐mediated immunity to various pathogens.
细胞因子刺激的人间皮细胞对中性粒细胞（包括 NAP-1/IL-8）产生趋化活性。
DOI：
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发表时间：
1992
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Goodman,RB;Wood,RG;Martin,TR;Hanson-Painton,O;Kinasewitz,GT
通讯作者：
Kinasewitz,GT