ALL YOU WANTED TO KNOW ABOUT SELEX
ALL YOU WANTED TO KNOW ABOUT SELEX
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DOI：
10.1007/bf00996358
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发表时间：
1994-01-01
影响因子：
2.8
通讯作者：
FAMULOK, M
中科院分区：
文献类型：
--
作者：
KLUG, SJ;FAMULOK, M
体外选择，或SELEX，是一种允许同时筛选高度不同的不同RNA或DNA(dsDNA或ssDNA)分子池中特定特征的技术。描述了体外选择实验的各种应用的不同例子，并对该方法及其变体进行了详细的概述。详细讨论了一些特别有说服力的体外选择实验，以说明该方法的潜在能力和多样性。指出了这些方法的潜在局限性和可能的克服途径。
In vitro selection, or SELEX, is a technique that allows the simultaneous screening of highly diverse pools of different RNA or DNA (dsDNA or ssDNA) molecules for a particular feature. Different examples from a great variety of applications of in vitro selection experiments are described and a detailed overview of the method and its variations will be given. Some especially conclusive in vitro selection experiments are discussed in detail to illustrate the potential power and diversity of this method. Potential restrictions of the methods and possible ways to overcome them are pointed out.