Cytological and functional characteristics of fascia adipocytes in rats: A unique population of adipocytes
Cytological and functional characteristics of fascia adipocytes in rats: A unique population of adipocytes
复制标题
大鼠筋膜脂肪细胞的细胞学和功能特征：独特的脂肪细胞群
DOI：
10.1016/j.bbalip.2019.158585
复制
发表时间：
2020-02-01
影响因子：
4.8
通讯作者：
Xu, Guoheng
中科院分区：
文献类型：
--
作者：
Zhang, Yanfei;Su, Xueying;Xu, Guoheng
真皮脂肪细胞、浅筋膜和皮下脂肪组织（SAT）存在于真皮和肌筋膜之间的间隙。它们彼此相邻，传统上被认为是一个SAT。最近，真皮脂肪细胞被重新定义为独立于SAT的独特群体。此外，我们在大鼠浅筋膜中发现了一种新型的脂肪形成祖细胞。本研究旨在探讨大鼠筋膜脂肪细胞的细胞学和功能特征。新生大鼠浅筋膜未见脂肪细胞，生长大鼠逐渐出现大量脂肪细胞。在体外实验中发现，成脂祖细胞位于筋膜内，具有较强的自发和诱导成脂能力。分化的筋膜脂肪细胞与皮下或内脏脂肪细胞相比，脂肪甘油三酯脂肪酶表达增加，但β -肾上腺素受体、滨利平-1和激素敏感脂肪酶（HSL）表达减少，因此具有高基础脂解，但对儿茶酚胺的脂解反应低。在儿茶酚胺而非肾上腺受体后脂溶刺激剂的作用下，滨利平-1和HSL的磷酸化以及HSL向脂滴的易位被减弱。结果提示浅筋膜是脂肪细胞的来源，具有明显的发育、细胞学和功能特征。我们提出筋膜脂肪细胞可以被认为是体内独特的脂肪细胞群。脂肪细胞的筋膜起源作为一种脂肪形成模型，可以从逻辑上解释脂肪新生发生在原本不存在脂肪组织的解剖位置，因此没有脂肪来源的间质前体。此外，还讨论了真皮、浅筋膜、SAT及其脂肪细胞之间的特殊组织解剖关系和重叠。
The dermal adipocytes, superficial fascia and subcutaneous adipose tissue (SAT) exist in the interspaces between the dermis and muscular fascia. They are adjacent to each other and traditionally recognized as one SAT. Recently, the dermal adipocyte was redefined as a unique population independent from the SAT. Also, we identified a novel type of adipogenic progenitors in rat superficial fascia. This study aimed to examine cytological and functional characteristics of fascial adipocytes in rats. Superficial fascia had no adipocytes in neonatal rats but gradually appeared numbers of adipocytes in growing rats. Adipogenic progenitors were found to reside in fascia and had strong ability in spontaneous and induced adipogenic differentiation in vitro. Differentiated fascial adipocytes versus subcutaneous or visceral adipocytes expressed increased adipose triglyceride lipase but decreased beta-adrenoreceptor, perilipin-1 and hormone-sensitive lipase (HSL), thus having high basal lipolysis but low lipolysis response to catecholamines. Phosphorylation of perilipin-1 and HSL and translocation of HSL to lipid droplets were attenuated in response to catecholamines rather than post-adrenoreceptoral lipolytic stimulators. The results suggested that superficial fascia was an origin of adipocytes with distinct developmental, cytological and functional characteristics. We proposed that fascial adipocytes could be considered as a unique population of adipocytes in the body. The fascia origin of adipocytes as an adipogenic model might logically explain fat neogenesis occurred at anatomical locations where originally exist no adipose tissues and thereby no adipose-derived stromal precursors. Also, the special histoanatomical relations and overlaps between the dermis, superficial fascia, SAT, and their adipocytes were discussed.