Tumor-Associated Macrophages in Pediatric Classical Hodgkin Lymphoma: Association with Epstein-Barr Virus, Lymphocyte Subsets, and Prognostic Impact
Tumor-Associated Macrophages in Pediatric Classical Hodgkin Lymphoma: Association with Epstein-Barr Virus, Lymphocyte Subsets, and Prognostic Impact
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DOI：
10.1158/1078-0432.ccr-12-0129
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发表时间：
2012-07-15
影响因子：
11.5
通讯作者：
Niedobitek, Gerald
中科院分区：
文献类型：
--
作者：
Barros, Mario Henrique M.;Hassan, Rocio;Niedobitek, Gerald
目的：肿瘤浸润性巨噬细胞与成人经典霍奇金淋巴瘤（cHL）的不良预后相关。我们之前已经显示了儿童cHL淋巴细胞组成的年龄相关变化。因此，我们假设儿童cHL中巨噬细胞的数量、功能和预后影响与成人病例不同。实验设计：应用免疫组织化学方法分析小儿cHL肿瘤微环境中巨噬细胞和树突状细胞（DC）的数量。结果分析了年龄、组织学特征、eb病毒（EBV）状态、临床随访和我们之前对这些病例的t细胞群的研究。结果：100例cHL病例中，结节性硬化占69%，混合细胞性病变占23%。ebv阳性占44.8%。患者= 2例（P = 0.007）。大量CD163（+）细胞与较差的无进展生存相关（PFS; P = 0.015）。此外，高数量的CD163（+）和颗粒酶B+细胞与ebv阴性患者的PFS恶化相关（P = 0.005），而与ebv阳性患者无关。结论：我们的研究结果表明，儿童cHL的巨噬细胞组成与成人不同。巨噬细胞的功能状态及其作为儿童cHL预后指标的价值可能取决于EBV状态。临床癌症研究；18日(14);3762 - 71。AACR (C) 2012。
Purpose: Tumor-infiltrating macrophages are associated with adverse outcome in adult classical Hodgkin lymphoma (cHL). We have previously shown age-related changes in the lymphocyte composition of pediatric cHL. We therefore hypothesized that the number, function, and prognostic impact of macrophages in pediatric cHL would be different from adult cases.Experimental Design: We analyzed the number of macrophages and dendritic cells (DC) in the tumor microenvironment of pediatric cHL by immunohistochemistry.Results were analyzed in context of age, histologic characteristics, Epstein-Barr virus (EBV) status, clinical follow-up, and our previous study of T-cell populations in these cases. Results: One hundred cHL cases were studied, including 69% nodular sclerosis and 23% mixed cellularity cases. A total of 44.8% of cases were EBV-positive. Patients = 2 (P = 0.007). High numbers of CD163(+) cells were associated with worse progression-free survival (PFS; P = 0.015). Furthermore, high numbers of CD163(+) and granzyme B+ cells were associated with worse PFS in EBV-negative (P = 0.005) but not in EBV-positive cases.Conclusion: Our results suggest that macrophage composition in pediatric cHL is distinct from adults. Functional status of macrophages and their value as prognostic indicators in pediatric cHL may depend on EBV status. Clin Cancer Res; 18(14); 3762-71. (C)2012 AACR.