The orphan COUP-TF nuclear receptors are markers for neurogenesis from cnidarians to vertebrates
The orphan COUP-TF nuclear receptors are markers for neurogenesis from cnidarians to vertebrates
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DOI：
10.1016/j.ydbio.2004.07.037
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发表时间：
2004-11-01
影响因子：
2.7
通讯作者：
Galliot, B
中科院分区：
文献类型：
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作者：
Gauchat, D;Escriva, H;Galliot, B
在双侧性，COUP-TF核受体参与神经发生和/或中枢神经系统模式。在水螅中，神经系统由感觉机械感受器细胞（神经细胞）和神经元细胞形成，这两种谱系都源自共同的干细胞。水螅COUP-TF基因（hyCOUP-TF）属于单系COUP-TF孤儿受体家族（NR2F），编码高度保守的DNA结合域和配体结合域。在成人息肉中，hyCOUP-TF在成纤维细胞和一部分神经元细胞中表达。对表达hyCOUP-TF或配对样基因prdl-b的细胞进行的比较BrDU标记分析表明，prdl-b表达对应于增殖的早期阶段，而hyCOUP-TF检测稍晚。表达HyCOUP-TF和prdl-b的细胞在sf-1突变体中消失，变成"无神经"。此外，hyCOUP-TF和prdl-b表达被排除在经历发育过程的区域之外。这些数据表明，hyCOUP-TF和prdl-b属于一个遗传网络，出现在早期后生动物进化过程中与神经发生。hyCOUP-TF蛋白特异性结合到进化上保守的DR1和DR5反应元件上，并且当在哺乳动物细胞中表达时，以剂量依赖性方式抑制RAR：RXR核受体诱导的反式激活。因此，刺胞动物的转录因子可以在脊椎动物细胞中是活跃的，这意味着COUP-TF和其他核受体之间的功能相互作用是进化保守的。(C)2004年爱思唯尔公司一切权利保留。
In bilaterians, COUP-TF nuclear receptors participate in neurogenesis and/or CNS patterning. In hydra, the nervous system is formed of sensory mechanoreceptor cells (nematocytes) and neuronal cells, both lineages deriving from a common stem cell. The hydra COUP-TF gene, hyCOUP-TF, which encodes highly conserved DNA-binding and ligand-binding domains, belongs to the monophyletic COUP-TFs orphan receptor family (NR2F). In adult polyps, hyCOUP-TF is expressed in nematoblasts and a subset of neuronal cells. Comparative BrDU labeling analyses performed on cells expressing either hyCOUP-TF or the paired-like gene prdl-b showed that prdl-b expression corresponded to early stages of proliferation, while hyCOUP-TF was detected slightly later. HyCOUP-TF and prdl-b expressing cells disappeared in sf-1 mutants becoming "nerve-free". Moreover hyCOUP-TF and prdl-b expression was excluded from regions undergoing developmental processes. These data suggest that hyCOUP-TF and prdl-b belong to a genetic network that appeared together with neurogenesis during early metazoan evolution. The hyCOUP-TF protein specifically bound onto the evolutionarily conserved DR1 and DR5 response elements, and repressed transactivation induced by RAR:RXR nuclear receptors in a dose-dependent manner when expressed in mammalian cells. Hence, a cnidarian transcription factor can be active in vertebrate cells, implying that functional interactions between COUP-TF and other nuclear receptors were evolutionarily conserved. (C) 2004 Elsevier Inc. All rigths reserved.