Allicin protects porcine oocytes against LPS-induced defects during maturation in vitro
Allicin protects porcine oocytes against LPS-induced defects during maturation in vitro
复制标题
大蒜素保护猪卵母细胞在体外成熟过程中免受脂多糖诱导的缺陷
DOI：
10.1016/j.theriogenology.2022.02.007
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发表时间：
2022-02-14
期刊：
影响因子：
2.8
通讯作者：
Yang, Xiaogan
中科院分区：
文献类型：
--
作者：
Li, Qiqi;Zhang, Yaping;Yang, Xiaogan
脂多糖（LPS）是革兰氏阴性菌的主要毒力因子，严重损害女性生殖系统的功能。它对卵母细胞和随后的胚胎发育特别有害。利用植物活性物质来改善LPS引起的损伤是一个值得关注的策略。本研究以猪卵母细胞为研究对象，探讨大蒜素对脂多糖（LPS）暴露的猪卵母细胞的保护作用及其机制。我们的数据表明，1 mM大蒜素的补充显着衰减LPS介导的第一极体挤出率和随后的囊胚形成率的降低。大蒜素还减轻了由LPS暴露引起的纺锤体组装、染色体排列、肌动蛋白聚合和皮质颗粒分布的异常。此外，大蒜素恢复活性氧（ROS），早期细胞凋亡和自噬的正常生理水平在LPS暴露的卵母细胞。总之，我们的研究结果证实，大蒜素可以保护卵母细胞免受LPS诱导的损伤。本研究结果将有助于促进植物源性生物活性物质在改善卵母细胞成熟缺陷方面的应用。(c)2022爱思唯尔公司All rights reserved.
Lipopolysaccharide (LPS), the main virulence factor of gram-negative bacteria, severely impairs the function of the female reproductive system. It has especially harmful effects on oocytes and subsequent embryonic development. The use of active plant substances to ameliorate the damage caused by LPS exposure is a strategy worthy of attention. In this study, porcine oocytes were used to investigate the protective effects and underlying mechanisms of allicin, an extract derived from garlic, on LPS-exposed oocytes in vitro. Our data indicated that supplementation with 1 mM allicin significantly attenuated the LPS-mediated reductions in the first polar body extrusion rate and the subsequent blastocyst formation rate. Allicin also mitigated the abnormalities in spindle assembly, chromosome alignment, actin polymerization, and cortical granule distribution caused by LPS exposure. Furthermore, allicin restored reactive oxygen species (ROS), early apoptosis and autophagy to normal physiological levels in LPS-exposed oocytes. In conclusion, our findings confirm that allicin can protect oocytes against LPS-induced damage. The results of this study will help promote the application of plant-derived bioactive substances to ameliorate oocyte maturation defects. (c) 2022 Elsevier Inc. All rights reserved.