Perinatal exposure to bisphenol A increases in the adulthood of the offspring the susceptibility to the human parasite Toxocara canis
Perinatal exposure to bisphenol A increases in the adulthood of the offspring the susceptibility to the human parasite Toxocara canis
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DOI：
10.1016/j.envres.2020.109381
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发表时间：
2020-05-01
影响因子：
8.3
通讯作者：
Morales-Montor, Jorge
中科院分区：
文献类型：
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作者：
Del Rio-Araiza, Victor H.;Palacios-Arreola, Margarita, I;Morales-Montor, Jorge
双酚A是一种广泛存在的环境污染物和内分泌干扰物，可与多种类固醇受体，特别是雌激素受体相互作用。在不同的研究中，人们观察到，在发育的关键时期内分泌失调可能会引发成年期免疫反应的改变。雄性Wistar大鼠在围产期（从妊娠第5天到出生后第21天）以250 μ g/kg/天的剂量间接暴露于BPA，在60天龄时，成年后，用犬弓首线虫的幼虫卵感染动物，并在感染后7天处死。通过人工消化分析肺和肝中的寄生虫负荷。此外，通过流式细胞术分析了脾脏、外周和肠系膜淋巴结中存在的免疫细胞亚群（巨噬细胞、NK细胞、Ty δ、总T细胞、辅助性T细胞、细胞毒性T细胞和B淋巴细胞）。实时定量PCR检测脾脏Th 1和Th 2细胞因子的表达。最后用ELISA法检测血清中抗疟原虫特异性抗体的效价。在围产期给予BPA处理有利于在成年期肺和肝中的犬弓蛔虫幼虫百分比的显著增加。此外，暴露于该化合物导致针对该寄生虫的特异性抗体的产生显著减少，同时下调Th 2细胞因子（IL-4、IL-5和IL-13），同时上调Th 1细胞因子（IFN-γ和TNF-α）。围产期暴露于BPA会影响成年期免疫反应的表现，改变这些细胞产生的细胞因子和抗体，这有利于感染的易感性，特别是弓形虫病。
Bisphenol A, a very widespread environmental pollutant and endocrine disruptor compound, can interact with several steroid receptors, particularly with estrogen ones. In different studies, it has observed that the endocrine disruption during critical periods of development can trigger alterations in the immune response during the adult life. Male Wistar rats were exposed indirectly to BPA at a dose of 250 mu g/kg day during the perinatal period (from day 5 of pregnancy until day 21 postnatal), M the 60 days of age, the adulthood, animals were infected with larvated eggs of the Toxocara canis, and were sacrificed at 7 days post-infection. Parasitic loads in the lung and in the liver were analyzed by artificial digestion. Furthermore, immune cell subpopulations (macrophages, NK cells, Ty delta, total T cells, T helper, T cytotoxic, and B lymphocytes) present in spleen, peripheral and mesenteric lymph nodes were analyzed by flow cytometry. The expression of Th1 and Th2 cytokines at the splenic level was determined by real-time quantitative PCR. Finally, the titers of specific antibodies against to the parasite were analyzed by ELISA. The BPA treatment administrated in the perinatally stage favors a significant increase of the percentage of Toxocara canis larvae in the lungs and liver in the adulthood. Additionally, the exposure to this compound caused a dramatically decrease in the production of specific antibodies against to this parasite, downregulating together Th2 cytokines (IL-4, IL-5 and IL-13), meanwhile upregulated Th1 cytokines (IFN-gamma and TNF-alpha). Perinatal exposure to BPA affects the performance of the immune response during adult life, modifying both cytokines and antibodies production by these cells, which favors the susceptibility to infections, specifically toxocariosis.