GADD45 proteins inhibit HIV-1 replication through specific suppression of HIV-1 transcription
GADD45 proteins inhibit HIV-1 replication through specific suppression of HIV-1 transcription
复制标题
GADD45 蛋白通过特异性抑制 HIV-1 转录来抑制 HIV-1 复制
DOI：
10.1016/j.virol.2016.02.014
复制
发表时间：
2016-06-01
期刊：
影响因子：
3.7
通讯作者：
Qiao, Wentao
中科院分区：
文献类型：
--
作者：
Liang, Zhibin;Liu, Ruikang;Qiao, Wentao
GADD 45蛋白是一类应激诱导蛋白，参与细胞周期调控、细胞存活和死亡、DNA修复和去甲基化等多种细胞通路。最近发现HIV-1感染诱导GADD 45蛋白的表达。然而，GADD 45对HIV-1复制的影响尚未研究。在这里，我们报告GADD 45蛋白的过表达通过抑制HIV-1 LTR启动子的转录来减少HIV-1的产生。这种抑制作用对HIV-1是特异性的，因为GADD 45蛋白既不抑制来自其他逆转录病毒的LTR启动子，也不减少这些病毒的产生。内源性GADD 45的敲低会适度激活J-Lat A72潜伏细胞系中的HIV-1，这表明GADD 45蛋白可能在维持HIV-1潜伏期方面发挥作用。(C)2016 Elsevier Inc. All rights reserved.
GADD45 proteins are a group of stress-induced proteins and participate in various cellular pathways including cell cycle regulation, cell survival and death, DNA repair and demethylation. It was recently shown that HIV-1 infection induces the expression of GADD45 proteins. However, the effect of GADD45 on HIV-1 replication has not been studied. Here, we report that overexpression of GADD45 proteins reduces HIV-1 production through suppressing transcription from the HIV-1 LTR promoter. This inhibitory effect is specific to HIV-1, since GADD45 proteins neither inhibit the LTR promoters from other retroviruses nor reduce the production of these viruses. Knockdown of endogenous GADD45 modestly activates HIV-1 in the J-Lat A72 latency cell line, which suggests GADD45 proteins might play a role in maintaining HIV-1 latency. (C) 2016 Elsevier Inc. All rights reserved.