Novel bioresources for studies of Brassica oleracea: identification of a kale MYB transcription factor responsible for glucosinolate production
Novel bioresources for studies of Brassica oleracea: identification of a kale MYB transcription factor responsible for glucosinolate production
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DOI：
10.1111/pbi.12095
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发表时间：
2013-10-01
影响因子：
13.8
通讯作者：
Hirai, Masami Yokota
中科院分区：
文献类型：
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作者：
Araki, Ryoichi;Hasumi, Akiko;Hirai, Masami Yokota
芥子油苷（GSL）是一种抗病原体和昆虫的防御化合物，在菊科植物中具有物种特异性。GSL还表现出各种促进人类健康的特性。其中，萝卜硫苷（脂肪族4-甲基亚磺酰基丁基GSL）引起了最多的关注，因为它水解形成一种有效的抗癌化合物。对开发具有理想GSL谱的菊科作物的商业品种的兴趣增加，导致尝试鉴定对控制GSL生物合成具有潜在价值的基因。然而，羽衣甘蓝（Brassica oleracea var. acephala）。在这项研究中，我们建立了全长羽衣甘蓝cDNA文库包含59904个克隆，这是用来产生一个表达序列标签（EST）数据集与119204条目。EST数据集明确了羽衣甘蓝中GSL生物合成途径相关基因。我们特别关注BoMYB 29，拟南芥MYB 29/PMG 2/HAG 3的同源物，不仅要表征其功能，还要证明其作为生物资源的可用性。BoMYB 29在野生型拟南芥中的过表达增强了脂肪族GSL生物合成基因的表达和脂肪族GSL的积累。当在myb 28 myb 29突变体中表达时，其没有表现出可检测到的脂肪族GSL，BoMYB 29恢复了生物合成基因的表达和脂肪族GSL积累。有趣的是，甲基亚磺酰基GSL含量（包括硫代葡萄糖苷）与甲硫基GSL含量的比率大大增加，表明BoMYB 29适合作为增加甲基亚磺酰基GSL含量的调节剂。我们的研究结果表明，这些生物资源可以促进进一步鉴定基因的GSL配置文件的修改和积累羽衣甘蓝有用。
Plants belonging to the Brassicaceae family exhibit species-specific profiles of glucosinolates (GSLs), a class of defence compounds against pathogens and insects. GSLs also exhibit various human health-promoting properties. Among them, glucoraphanin (aliphatic 4-methylsulphinylbutyl GSL) has attracted the most attention because it hydrolyses to form a potent anticancer compound. Increased interest in developing commercial varieties of Brassicaceae crops with desirable GSL profiles has led to attempts to identify genes that are potentially valuable for controlling GSL biosynthesis. However, little attention has been focused on genes of kale (Brassica oleracea var. acephala). In this study, we established full-length kale cDNA libraries containing 59904 clones, which were used to generate an expressed sequence tag (EST) data set with 119204 entries. The EST data set clarified genes related to the GSL biosynthesis pathway in kale. We specifically focused on BoMYB29, a homolog of Arabidopsis MYB29/PMG2/HAG3, not only to characterize its function but also to demonstrate its usability as a biological resource. BoMYB29 overexpression in wild-type Arabidopsis enhanced the expression of aliphatic GSL biosynthetic genes and the accumulation of aliphatic GSLs. When expressed in the myb28myb29 mutant, which exhibited no detectable aliphatic GSLs, BoMYB29 restored the expression of biosynthetic genes and aliphatic GSL accumulation. Interestingly, the ratio of methylsulphinyl GSL content, including glucoraphanin, to that of methylthio GSLs was greatly increased, indicating the suitability of BoMYB29 as a regulator for increasing methylsulphinyl GSL content. Our results indicate that these biological resources can facilitate further identification of genes useful for modifications of GSL profiles and accumulation in kale.