Cortical and trabecular bone benefits of mechanical loading are maintained long term in mice independent of ovariectomy.
Cortical and trabecular bone benefits of mechanical loading are maintained long term in mice independent of ovariectomy.
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DOI：
10.1002/jbmr.2143
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发表时间：
2014
影响因子：
6.2
通讯作者：
Fuchs, Robyn K.
中科院分区：
文献类型：
--
作者：
Warden, Stuart J.;Galley, Matthew R.;Hurd, Andrea L.;Richard, Jeffrey S.;George, Lydia A.;Guildenbecher, Elizabeth A.;Barker, Rick G.;Fuchs, Robyn K.
骨骼负荷增强了皮质骨和松质骨的特性。这些好处在停止负荷后能持续多久仍然是一个悬而未决的临床相关问题。这项研究调查了长期维持负荷诱导的雌性C57BL/6小鼠皮质和骨小梁的益处，以及手术诱导的绝经对维持的影响。采用小鼠胫骨轴向压缩加载模型，对16周龄动物的右侧胫骨施加外力，每周3天，连续4周。左侧胫骨不加载，作为内对照。随后对动物解除训练(限制在笼子活动中)0、4、8、26或52周，在解除训练0周进行卵巢切除(OVX)或假去卵巢手术。负荷增加了胫骨中段的皮质骨量、大小和强度，以及胫骨近端的骨体积分数。在26周的训练中，由于骨髓的高度扩张，皮质骨量、面积和厚度在负荷中的优势消失了。然而，负荷对骨骼总面积和强度的益处在每个去训练时间点都保持不变。同样，负荷对骨体积分数的好处在所有解除训练的时间点都持续存在。皮质骨和松质骨负荷的长期益处不受手术引起的绝经的影响，因为负荷和手术之间没有相互作用。但OVX对大鼠早期(4周和8周)的皮质骨特性和各时间点的骨小梁特性均有独立的影响。这些累积数据表明，负荷对皮质骨的大小和强度(但不是质量)和骨小梁形态有长期的好处，这些都不受手术诱导的更年期的影响。这表明，与体力活动相关的骨骼负荷可能会通过准备骨骼来抵消与衰老和更年期相关的皮质和骨小梁变化，从而提供长期的好处。
Skeletal loading enhances cortical and trabecular bone properties. How long these benefits last following loading cessation remains an unresolved, clinically-relevant question. This study investigated long-term maintenance of loading-induced cortical and trabecular bone benefits in female C57BL/6 mice and the influence of a surgically-induced menopause on the maintenance. 16-week-old animals had their right tibia extrinsically loaded 3 days/week for 4 weeks using the mouse tibial axial compression loading model. Left tibias were not loaded and served as internal controls. Animals were subsequently detrained (restricted to cage activities) for 0, 4, 8, 26 or 52 weeks, with ovariectomy (OVX) or sham-OVX surgery being performed at 0 weeks detraining. Loading increased midshaft tibia cortical bone mass, size and strength, and proximal tibia bone volume fraction. The cortical bone mass, area and thickness benefits of loading were lost by 26 weeks of detraining due to heightened medullary expansion. However, loading-induced benefits on bone total area and strength were maintained at each detraining time point. Similarly, the benefits of loading on bone volume fraction persisted at all detraining time points. The long-term benefits of loading on both cortical and trabecular bone were not influenced by a surgically-induced menopause as there were no interactions between loading and surgery. However, OVX had independent effects on cortical bone properties at early (4 and 8 weeks) detraining time points and trabecular bone properties at all detraining time points. These cumulative data indicate loading has long-term benefits on cortical bone size and strength (but not mass) and trabecular bone morphology which are not influenced by a surgically-induced menopause. This suggests skeletal loading associated with physical activity may provide long-term benefits by preparing the skeleton to offset both the cortical and trabecular bone changes associated with aging and menopause.
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影响因子：
6.2
作者：
Järvinen, TLN;Pajamäki, I;Kannus, P
通讯作者：
Kannus, P
影响因子：
6.2
作者：
Bass, SL;Saxon, L;Stuckey, S
通讯作者：
Stuckey, S
影响因子：
--
作者：
Poulet, Blandine;Hamilton, Richard W.;Pitsillides, Andrew A.
通讯作者：
Pitsillides, Andrew A.
影响因子：
6.2
作者：
Gunter, Katherine;Baxter-Jones, Adam D. G.;Snow, Christine
通讯作者：
Snow, Christine
影响因子：
4.1
作者：
De Souza, RL;Matsuura, M;Pitsillides, AA
通讯作者：
Pitsillides, AA