Long range synchronization within the enteric nervous system underlies propulsion along the large intestine in mice.
Long range synchronization within the enteric nervous system underlies propulsion along the large intestine in mice.
复制标题
肠神经系统内的长距离同步是小鼠沿大肠沿着推进的基础。
DOI：
10.1038/s42003-021-02485-4
复制
发表时间：
2021-08-10
影响因子：
5.9
通讯作者：
Sorensen J
中科院分区：
文献类型：
--
作者：
Spencer NJ;Travis L;Wiklendt L;Costa M;Hibberd TJ;Brookes SJ;Dinning P;Hu H;Wattchow DA;Sorensen J
肠神经系统（ENS）如何协调内容物沿着胃肠道（GI）的推进一直是一个主要的未解决的问题。我们揭示了一种机制，解释了ENS活动的基础推进内容沿着结肠。我们使用了最近开发的高分辨率视频成像方法，同时从平滑肌电生理记录，在流体推进。记录显示，不仅在近端结肠，而且在远端结肠，早在传播收缩侵入远端区域之前，兴奋性和抑制性神经肌肉输入的脉动放电。在推进过程中，小波分析显示，增加相干性在2 Hz以上的近端和远端区域之间的大距离。因此，在推进过程中，下行抑制性神经通路在长范围内的同步放电会抑制平滑肌收缩，从而抵消结肠同一区域的兴奋性神经通路。这延迟了下游的肌肉收缩，在前进的收缩之前。确定的机制比预期的更复杂，并且与沿着其他中空平滑肌器官的流体推进沿着有很大不同;如淋巴管，门静脉或输尿管，它们在没有内在神经元的情况下进化。Nick Spencer等人利用离体小鼠结肠的结肠壁运动的视频成像进行了同时的多部位电生理记录，以便将内容物的推进与来自平滑肌的潜在电信号相关联。他们的研究结果表明，兴奋性和抑制性连接电位在近端和远端结肠中是同步的，这表明肠神经系统网络的通信范围比以前预期的要长。
How the Enteric Nervous System (ENS) coordinates propulsion of content along the gastrointestinal (GI)-tract has been a major unresolved issue. We reveal a mechanism that explains how ENS activity underlies propulsion of content along the colon. We used a recently developed high-resolution video imaging approach with concurrent electrophysiological recordings from smooth muscle, during fluid propulsion. Recordings showed pulsatile firing of excitatory and inhibitory neuromuscular inputs not only in proximal colon, but also distal colon, long before the propagating contraction invades the distal region. During propulsion, wavelet analysis revealed increased coherence at ~2 Hz over large distances between the proximal and distal regions. Therefore, during propulsion, synchronous firing of descending inhibitory nerve pathways over long ranges aborally acts to suppress smooth muscle from contracting, counteracting the excitatory nerve pathways over this same region of colon. This delays muscle contraction downstream, ahead of the advancing contraction. The mechanism identified is more complex than expected and vastly different from fluid propulsion along other hollow smooth muscle organs; like lymphatic vessels, portal vein, or ureters, that evolved without intrinsic neurons. Nick Spencer et al. made simultaneous multi-site electrophysiological recordings with video imaging of colonic wall movements from ex vivo mouse colon, in order to correlate propulsion of content with underlying electrical signals from the smooth muscle. Their results demonstrate that excitatory and inhibitory junction potentials are synchronized in both the proximal and distal colon, suggesting that the enteric nervous system network communicates over a longer range than previously expected.