Dynamic quantitative trait loci for salt stress components on chromosome 1 of rice
Dynamic quantitative trait loci for salt stress components on chromosome 1 of rice
复制标题
DOI：
10.1071/fp09247
复制
发表时间：
2010-01-01
影响因子：
3
通讯作者：
Steele, Katherine A.
中科院分区：
文献类型：
--
作者：
Haq, Tanveer Ul;Gorham, John;Steele, Katherine A.
水稻品种 Co39 和 Moroberekan 在中等盐度（100-150 mol m(-3) NaCl；10 : 1 或 20 : 1 Na+ 与 Ca2+ 比例）下生长时，叶片 Na+ 浓度不同。来自它们之间杂交的重组自交系（RIL）被用来绘制几周内盐胁迫下数量性状基因座（QTL）的图谱。两项实验（使用 170 和 96 个 RIL 以及 126 个 RFLP 标记的连锁图进行）确定了 1 号染色体上对应 11.07-14.6 Mbp 的区域中叶 Na+ 浓度和 K+ : Na+ 比率对 QTL 的主要影响。没有检测到叶C1-QTL。在第三个实验中，在 100 mol m(-3) 氯化钠浓度下 7 和 21 天后收获叶和叶鞘。通过添加 28 个微卫星标记改进了 1 号染色体的连锁图，这些标记解析了 Na+ 和 K+ 浓度以及 K+ : Na+ 比率的不同 QTL。胁迫7天后，最显着的QTL位于11.56-12.66 Mbp区域。最高的 Na+ 浓度记录在鞘中。检测到叶的 Na+ 浓度 QTL，但未检测到鞘的 Na+ 浓度。胁迫21天后，叶和鞘中含有最显着QTL的区域延伸至11.07 Mbp。在 11.39-12.39 Mbp 处发现了叶 Na+ 与鞘 Na+ 浓度比率的 QTL。这些发现表明，该区域的多个基因参与对盐度的响应，并且它们的影响根据胁迫持续时间、叶龄和类型而动态变化。
Rice varieties Co39 and Moroberekan differ for leaf Na+ concentrations when grown at moderate salinity (100-150 mol m(-3) NaCl; 10 : 1 or 20 : 1 Na+ to Ca2+ ratio). Recombinant inbred lines (RILs) from a cross between them were used to map quantitative trait loci (QTL) under salt stress over several weeks. Two experiments (conducted with 170 and 96 RILs, and a linkage map of 126 RFLP markers) identified a major effect on QTL for leaf Na+ concentration and K+ : Na+ ratio on chromosome 1 in a region corresponding to 11.07-14.6 Mbp. No leaf Cl- QTL were detected. In a third experiment, leaves and sheaths were harvested after 7 and 21 days at 100 mol m(-3) NaCl. The linkage map of chromosome 1 was improved by the addition of 28 microsatellite markers, which resolved distinct QTL for Na+ and K+ concentrations, and K+ : Na+ ratio. After 7 days' stress, the most significant QTL were in the region of 11.56-12.66 Mbp. The highest Na+ concentrations were recorded in the sheaths. Na+ concentrationQTLwere detected for leaves, but not for sheaths. After 21 days' stress, the region containing the most significant QTL extended to 11.07 Mbp in leaves and in sheaths. A QTL for the ratio of leaf Na+ to sheath Na+ concentrations was found at 11.39-12.39 Mbp. These findings suggest that multiple genes in this region are involved in the response to salinity, and their impact is dynamic according to stress duration, and leaf age and type.