Effects of green tea consumption on human fecal microbiota with special reference to Bifidobacterium species
Effects of green tea consumption on human fecal microbiota with special reference to Bifidobacterium species
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DOI：
10.1111/j.1348-0421.2012.00502.x
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发表时间：
2012-11-01
影响因子：
2.6
通讯作者：
Benno, Yoshimi
中科院分区：
文献类型：
--
作者：
Jin, Jong-Sik;Touyama, Mutsumi;Benno, Yoshimi
绿色茶是世界上最受欢迎的饮料之一。其有益健康的影响和成分已被广泛审查。然而，关于绿色茶消费对人体肠道微生物群（HIM）的影响知之甚少，HIM在人类健康中起着至关重要的作用。10名志愿者通常不喝绿色茶，喝了10天，然后停止喝7天。在三个时间点收集他们的粪便样本：开始10天绿茶方案之前，10天结束时，停止方案后7天。他们的粪便样本进行了分析，末端限制性片段长度多态性与特定的引物限制性内切酶系统HIM和双歧杆菌属的实时PCR方法。虽然每个受试者的HIM相对稳定，但双歧杆菌的种类比例在其粪便微生物群的分类中起着重要作用。尽管双歧杆菌属的种类存在个体间差异，但注意到由于绿色茶的消耗，双歧杆菌的比例总体上有增加的趋势。然而，在每个样品中观察到双歧杆菌属的组成几乎没有变化。这表明，比例的变化是诱导，而不是物种间的过渡，但物种内的增加和/或减少。总之，绿色茶消费可能作为益生元，并通过增加双歧杆菌物种的比例来改善结肠环境。
Green tea is one of the most popular beverages in the world. Its beneficial health effects and components have been extensively reviewed. However, little is known about the influence of green tea consumption on the human intestinal microbiota (HIM), which plays a crucial role in human health. Ten volunteers who did not usually consume green tea, drank it for 10 days and then stopped drinking it for 7 days. Their fecal samples were collected at three time points: before beginning the 10-day green-tea regime, at the conclusion of that 10 days, and 7 days after stopping the regime. Their fecal samples were analyzed by terminal restriction fragment length polymorphism with specific primer-restriction enzyme systems for HIM and by using a real-time PCR method for the Bifidobacterium species. Although the HIM of each subject was relatively stable, the proportion of Bifidobacterium species played an important role in the classification of their fecal microbiota. Although there were inter-individual differences in the Bifidobacterium species, an overall tendency for the proportion of bifidobacteria to increase because of green tea consumption was noted. However, little change was observed in the composition of Bifidobacterium species in each sample. This suggests that the change in proportion was induced, not by an inter-species transition, but by an intra-species increase and/or decrease. In conclusion, green tea consumption might act as a prebiotic and improve the colon environment by increasing the proportion of the Bifidobacterium species.