3D printing and modelling of customized implants and surgical guides for non-human primates.
3D printing and modelling of customized implants and surgical guides for non-human primates.
复制标题
DOI：
10.1016/j.jneumeth.2017.05.013
复制
发表时间：
2017-07-15
影响因子：
3
通讯作者：
Roelfsema PR
中科院分区：
文献类型：
--
作者：
Chen X;Possel JK;Wacongne C;van Ham AF;Klink PC;Roelfsema PR
我们为电生理学创造了负担得起的、定制的3D打印颅骨植入物。这些包括头柱，录音室，和基座基板。在计划和手术过程中还使用了比例模型和开颅术导向器。在手术过程中提高了动物福利，降低了感染风险。到目前为止，我们的植入物仍然坚固，并与头骨良好整合。灵长类神经生物学家使用长期植入的设备，如用于头部稳定的神经元和腔室，以进入大脑并研究其活动。这种植入物安装在颅骨上，由坚硬耐用的材料制成，如钛。在这里，我们提出了一种低成本的方法来创建定制的3D打印颅骨植入物，这些植入物是根据个体动物的解剖结构定制的。我们进行了术前计算机断层扫描（CT）和磁共振（MR）扫描，以生成颅骨和大脑的三维（3D）模型。然后，我们使用3D建模软件来设计可植入的头柱、腔室和基座锚固底座，以及开颅手术指南，以在手术过程中帮助我们。原型由塑料或树脂制成，而植入物则由钛制成。植入物经过后处理，并接受骨相容性材料涂层，以促进骨整合。它们的量身定制的配合极大地促进了外科植入，并消除了植入物和骨之间的差距。到目前为止，我们的植入物仍然坚固，并与头骨良好整合。商业现成的解决方案通常具有统一的平坦底座，防止它们与头骨的弯曲表面齐平。这就为液体和组织进入留下了间隙，增加了微生物感染和组织炎症以及植入物丢失的风险。3D打印技术的使用使我们能够快速、经济地创建独特、复杂的设计，避免了传统生产方法的限制，从而提高了实验的成功率，改善了动物的健康状况。
We created affordable, customized, 3D-printed cranial implants for electrophysiology. These included head posts, recording chambers, and pedestal base plates. Scale models and craniotomy guides were also used during planning and surgery. Animal welfare was enhanced during surgery and risk of infection reduced. To date, our implants remain robust and well-integrated with the skull. Primate neurobiologists use chronically implanted devices such as pedestals for head stabilization and chambers to gain access to the brain and study its activity. Such implants are skull-mounted, and made from a hard, durable material, such as titanium. Here, we present a low-cost method of creating customized 3D-printed cranial implants that are tailored to the anatomy of individual animals. We performed pre-surgical computed tomography (CT) and magnetic resonance (MR) scans to generate three-dimensional (3D) models of the skull and brain. We then used 3D modelling software to design implantable head posts, chambers, and a pedestal anchorage base, as well as craniotomy guides to aid us during surgery. Prototypes were made from plastic or resin, while implants were 3D-printed in titanium. The implants underwent post-processing and received a coating of osteocompatible material to promote bone integration. Their tailored fit greatly facilitated surgical implantation, and eliminated the gap between the implant and the bone. To date, our implants remain robust and well-integrated with the skull. Commercial-off-the-shelf solutions typically come with a uniform, flat base, preventing them from sitting flush against the curved surface of the skull. This leaves gaps for fluid and tissue ingress, increasing the risk of microbial infection and tissue inflammation, as well as implant loss. The use of 3D printing technology enabled us to quickly and affordably create unique, complex designs, avoiding the constraints levied by traditional production methods, thereby boosting experimental success and improving the wellbeing of the animals.