Visual tuning properties of genetically identified layer 2/3 neuronal types in the primary visual cortex of cre-transgenic mice.
Visual tuning properties of genetically identified layer 2/3 neuronal types in the primary visual cortex of cre-transgenic mice.
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DOI：
10.3389/fnsys.2010.00162
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发表时间：
2011
影响因子：
3
通讯作者：
Zeng H
中科院分区：
文献类型：
--
作者：
Zariwala HA;Madisen L;Ahrens KF;Bernard A;Lein ES;Jones AR;Zeng H
用记录微电极研究了小鼠初级视觉皮层V1内的兴奋性和抑制性细胞类别具有不同的功能特性。兴奋性神经元对移动光栅的取向角有较高的选择性，而假定的抑制性神经元对移动光栅的取向角有较差的选择性。然而，基因鉴定的中间神经元及其亚型的选择性研究仍然存在争议。在这里，我们使用新的cre驱动和报告小鼠来鉴定体内遗传亚群进行双光子钙染料成像：Wfs1(+)/Gad1（−）小鼠标记2/3层兴奋性细胞群，Pvalb(+)/Gad1（+）小鼠标记抑制性神经元遗传亚群。使用cre报告细胞系，两只小鼠的细胞被相同地标记为tdTomato蛋白，在体内可见。我们发现Wfs1（+）细胞表现出与兴奋性细胞相当的视觉调谐特性，即对定向移动光栅的角度、方向和空间频率具有高选择性和调谐。Pvalb（+）神经元的功能调谐与微电极研究中先前报道的窄峰中间神经元一致，表现出比兴奋性神经元更差的选择性。这项研究证明了cre转基因小鼠技术在选择性靶向神经元亚群中的应用，并使它们适合于结构、功能和连接研究。
The putative excitatory and inhibitory cell classes within the mouse primary visual cortex V1 have different functional properties as studied using recording microelectrode. Excitatory neurons show high selectivity for the orientation angle of moving gratings while the putative inhibitory neurons show poor selectivity. However, the study of selectivity of the genetically identified interneurons and their subtypes remain controversial. Here we use novel Cre-driver and reporter mice to identify genetic subpopulations in vivo for two-photon calcium dye imaging: Wfs1(+)/Gad1(−) mice that labels layer 2/3 excitatory cell population and Pvalb(+)/Gad1(+) mice that labels a genetic subpopulation of inhibitory neurons. The cells in both mice were identically labeled with a tdTomato protein, visible in vivo, using a Cre-reporter line. We found that the Wfs1(+) cells exhibited visual tuning properties comparable to the excitatory population, i.e., high selectivity and tuning to the angle, direction, and spatial frequency of oriented moving gratings. The functional tuning of Pvalb(+) neurons was consistent with previously reported narrow-spiking interneurons in microelectrode studies, exhibiting poorer selectivity than the excitatory neurons. This study demonstrates the utility of Cre-transgenic mouse technology in selective targeting of subpopulations of neurons and makes them amenable to structural, functional, and connectivity studies.