Liquid Chromatographic Isolation of Individual Amino Acids Extracted From Sediments for Radiocarbon Analysis
Liquid Chromatographic Isolation of Individual Amino Acids Extracted From Sediments for Radiocarbon Analysis
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DOI：
10.3389/fmars.2020.00174
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发表时间：
2020-03
期刊：
影响因子：
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通讯作者：
T M Blattmann;D. Montluçon;N. Haghipour;N. Ishikawa;T. Eglinton
中科院分区：
文献类型：
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作者：
T M Blattmann;D. Montluçon;N. Haghipour;N. Ishikawa;T. Eglinton
“生命的组成部分”以蛋白质、肽和单一氨基酸的形式在环境中几乎无处不在。为了阐明沉积环境中的氨基酸来源、循环和保存，我们提出了一种使用高压液相色谱分离从沉积物中提取的未衍生氨基酸的方法，以进行化合物特异性放射性碳分析。该方法由三个主要步骤组成，包括（1）用氢氟酸和盐酸提取氨基酸，然后脱盐，（2）使用两种互补的柱化学物质进行液相色谱分离和纯化，以及（3）通过加速器质谱进行后纯化和测量。该程序产生的空白估计含有 2.2 ± 1.3 μgC 和 0.25 ± 0.09 Fm。
The “building blocks of life” are found nearly ubiquitously in the environment in the form of proteins, peptides, and single amino acids. To shed light on amino acid sources, cycling, and preservation in sedimentary environments, we present a method using high-pressure liquid chromatography to separate and isolate underivatized amino acids extracted from sediments to conduct compound-specific radiocarbon analysis. This method consists of three main steps including (1) amino acid extraction by hydrofluoric and hydrochloric acids followed by desalting, (2) liquid chromatographic isolation and purification using two complementary column chemistries, and (3) post-purification and measurement by accelerator mass spectrometry. The resulting blank of this procedure is estimated to contain 2.2 ± 1.3 μgC with 0.25 ± 0.09 Fm.