Extracellular matrix formation and mineralization on a phosphate-free porous bioactive glass scaffold using primary human osteoblast (HOB) cells
Extracellular matrix formation and mineralization on a phosphate-free porous bioactive glass scaffold using primary human osteoblast (HOB) cells
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DOI：
10.1016/j.biomaterials.2006.11.022
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发表时间：
2007-03-01
期刊：
影响因子：
14
通讯作者：
Hench, Larry L.
中科院分区：
文献类型：
--
作者：
Jones, Julian R.;Tsigkou, Olga;Hench, Larry L.
溶胶-凝胶衍生的70 S30 C（70mol%SiO（2），30mol%CaO）组合物的生物活性玻璃已被发泡以产生具有类似于松质骨的分级互连孔形态的3D生物活性支架。本研究的目的是探讨原代人成骨细胞对多孔生物活性玻璃支架的反应。支架支持成骨细胞的生长和诱导分化，在3周的培养期内，如所示的增强的ALcase酶活性，而不添加补充因子，如抗坏血酸，β-甘油磷酸盐和地塞米松。这是第一次在不含磷酸盐的生物活性玻璃上观察到这种情况。细胞外基质的沉积也通过细胞外基质蛋白I型胶原蛋白的产生增强来证实。SEM显示在没有添加生长因子的情况下矿化骨结节形成的迹象。因此，70 S30 C生物活性玻璃支架满足骨组织工程应用理想支架的许多标准。(c)2006爱思唯尔有限公司保留所有权利。
Sol-get derived bioactive glasses of the 70S30C (70mol% SiO(2), 30mol% CaO) composition have been foamed to produce 3D bioactive scaffolds with hierarchical interconnected pore morphologies similar to trabecular bone. The aim of this study was to investigate primary human osteoblast response to porous bioactive glass scaffolds. The scaffolds supported osteoblast growth and induced differentiation, within the 3-week culture period, as depicted by enhanced ALPase enzymatic activity, without the addition of supplementary factors such as ascorbic acid, ss-glycerophosphate and dexamethasone. This is the first time this has been observed on a bioactive glass that does not contain phosphate. Deposition of extracellular matrix was also confirmed by enhanced production of the extracellular matrix protein collagen type I. SEM showed indications of mineralized bone nodule formation without the addition of growth factors. The 70S30C bioactive glass scaffolds therefore fulfil many of the criteria for an ideal scaffold for bone tissue engineering applications. (c) 2006 Elsevier Ltd. All rights reserved.