Atomic-force-controlled capillary electrophoretic nanoprinting of proteins
Atomic-force-controlled capillary electrophoretic nanoprinting of proteins
复制标题
蛋白质的原子力控制毛细管电泳纳米打印
DOI：
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发表时间：
2010
影响因子：
4.3
通讯作者：
E. Grushka
中科院分区：
文献类型：
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作者：
Yulia Lovsky;A. Lewis;C. Sukenik;E. Grushka
摘要：在非导电或导电基底上进行蛋白质的一般纳米打印和纳米注射，在位置和时间精度方面具有高度控制，是一个重要的目标，可能影响从生物技术（蛋白质芯片）到分子电子学，从细胞生物学基础研究到纳米光子学的各个领域。在本文中，我们将毛细管电泳（CE）（一种对蛋白质运动具有相当程度控制的分离方法）与原子力显微镜（AFM）无与伦比的位置精度相结合。这种组合提供了通过电泳或电渗将蛋白质迁移时间与 AFM 控制在确定位置和高度受限体积（估计为 2 al）高浓度蛋白质沉积相关联的能力。演示了标准蛋白质点样玻璃基板上牛血清白蛋白印刷的电气控制。为了实现这一进步，使用悬臂式玻璃锥形毛细管的钢笔纳米光刻（FPN）通过在纳米移液管本身上放置电极进行了改进。这导致施加的电压比毛细管电泳中通常使用的电压低三个数量级。原子力控制毛细管电泳印刷（ACCEP）的发展具有在时间和空间上都具有高分辨率的电泳分离的潜力。锥形纳米移液器尖端的大电压降使得小打印体积中的蛋白质浓度显着增加。所有这些属性结合起来表明这种方法应该对科学和技术产生重大影响。
图原子力控制电泳纳米打印 (ACCEP) 过程的图示（左）。毛细管电泳与原子力显微镜无与伦比的位置精度相结合，可以在非导电基底上对荧光标记的牛血清白蛋白进行高度受控的纳米打印（右图）
AbstractThe general nanoprinting and nanoinjection of proteins on non-conducting or conducting substrates with a high degree of control both in terms of positional and timing accuracy is an important goal that could impact diverse fields from biotechnology (protein chips) to molecular electronics and from fundamental studies in cell biology to nanophotonics. In this paper, we combine capillary electrophoresis (CE), a separation method with considerable control of protein movement, with the unparalleled positional accuracy of an atomic force microscope (AFM). This combination provides the ability to electrophoretically or electroosmotically correlate the timing of protein migration with AFM control of the protein deposition at a high concentration in defined locations and highly confined volumes estimated to be 2 al. Electrical control of bovine serum albumin printing on standard protein-spotting glass substrates is demonstrated. For this advance, fountain pen nanolithography (FPN) that uses cantilevered glass-tapered capillaries is amended with the placement of electrodes on the nanopipette itself. This results in imposed voltages that are three orders of magnitude less than what is normally used in capillary electrophoresis. The development of atomic-force-controlled capillary electrophoretic printing (ACCEP) has the potential for electrophoretic separation, with high resolution, both in time and in space. The large voltage drop at the tip of the tapered nanopipettes allows for significant increases in concentration of protein in the small printed volumes. All of these attributes combine to suggest that this methodology should have a significant impact in science and technology.
FigureDiagrammatic representation of the atomic-force-controlled electrophoretic nanoprinting (ACCEP) process, (on left). The combination of capillary electrophoresis with the unparalleled positional accuracy of an atomic force microscope allows for highly controlled nanoprinting of the fluorescently labelled protein bovine serum albumin on a non-conducting substrate (on right)