Three-dimensional microscopy with phase-shifting digital holography

Three-dimensional microscopy with phase-shifting digital holography
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DOI:
10.1364/ol.23.001221
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发表时间:
1998-08-01
期刊:
影响因子:
3.6
通讯作者:
Yamaguchi, I
Yamaguchi, I
中科院分区:
物理与天体物理2区
文献类型:
--
作者:
Zhang, T;Yamaguchi, I

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我们将相移数字全息术应用于显微镜,通过摄像机记录、相移分析和计算机重建,导出通过显微镜物镜从微观三维物体散射的光的复振幅。该方法不需要机械运动,并且提供了重建图像的灵活显示和定量评估。描述了图像形成的理论和样本的实验验证。 (C) 1998 年美国光学学会。
We applied phase-shifting digital holography to microscopy by deriving the complex amplitude of light scattered from microscopic three-dimensional objects through a microscope objective by video camera recording, phase-shifting analysis, and computer reconstruction. This method requires no mechanical movement and provides a flexible display and quantitative evaluation of the reconstructed images. A theory of image formation and experimental verification with specimens are described. (C) 1998 Optical Society of America.