EFFECT OF ASYNCHRONOUS SUPERINDUCTION ON EMBRYO SURVIVAL AND RANGE OF BLASTOCYST DEVELOPMENT IN SWINE
EFFECT OF ASYNCHRONOUS SUPERINDUCTION ON EMBRYO SURVIVAL AND RANGE OF BLASTOCYST DEVELOPMENT IN SWINE
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DOI：
10.1095/biolreprod35.1.133
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发表时间：
1986-08-01
影响因子：
3.6
通讯作者：
FIRST, NL
中科院分区：
文献类型：
--
作者：
POPE, WF;LAWYER, MS;FIRST, NL
通过比较异步超诱导（第6天胚胎转化为第7天妊娠受体和第7天胚胎转化为第6天妊娠受体）对第12天和第13天胚胎发育至随后存活至第30天的影响，研究了囊胚均匀发育的重要性。使用20头后备母猪，产生5只第7天受者接受第6天胚胎，5只第6天受者接受第7天胚胎。第7天和第6天受者的胚胎在6天后进行检查。恢复的胚胎形态从球形到丝状囊胚不等。这一胚胎范围在之前自然交配母猪所观察到的范围内。然而，从第6天胚胎移植到第7天受体的囊胚中恢复的囊胚在形态上更可变，并且比例上发育程度低于从第7天胚胎移植到第6天受体的囊胚。随后使用另外40头后备母猪产生20个受体（每个转移组10个受体），在第30天进行检查。移植至第6天受体的第7天胚胎存活率高于移植至第7天受体的第6天胚胎存活率（p < 0.05）。这些实验表明，胚胎早期发育的较大差异，在窝中，随后导致胚胎的更高死亡率。
The importance of uniform development of blastocysts was examined by comparing the effects of asynchronous superinduction (Day 6 embryos into Day 7 pregnant recipients and Day 7 embryos into Day 6 pregnant recipients) on the range of embryo development at Days 12 and 13 to subsequent survival to Day 30. Twenty gilts were used to produce five Day 7 recipients that received Day 6 embryos and five Day 6 recipients that received Day 7 embryos. Embryos from the Day 7 and Day 6 recipients were examined 6 days later. Recovered embryos ranged morphologically from spherical to filamentous blastocysts. This range of embryos was within the limits of that previously observed for naturally mated sows. However, recovered blastocysts from the Day 6 embryos transferred into Day 7 recipients were morphologically more variable and proportionately less developed than the blastocysts from the Day 7 embryos transferred into Day 6 recipients. Forty additional gilts were subsequently utilized to generate 20 recipients (10 recipients per transfer group) that were examined on Day 30. More Day 7 embroys transferred into Day 6 recipients survived (p < 0.05) than Day 6 embryos transferred into Day 7 recipients. These experiments suggested that greater variation in early development of embryos, within litters, subsequently resulted in greater mortality of embryos.