A single C4 Zinc finger-containing protein from Litopenaeus vannamei involved in antibacterial responses.
A single C4 Zinc finger-containing protein from Litopenaeus vannamei involved in antibacterial responses.
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来自凡纳滨对虾的单一 C4 含锌指蛋白参与抗菌反应。
DOI：
10.1016/j.fsi.2018.07.053
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Xu Xiaopeng
中科院分区：
文献类型：
--
作者：
Zuo Hongliang;Yang Linwei;Zheng Jiefu;Su Ziqi;Weng Shaoping;He Jianguo;Xu Xiaopeng
锌指结构域（ZnFs）广泛存在于许多蛋白质中，其含有由锌离子稳定的指状突起，并具有结合DNA、RNA、蛋白质和脂质底物的功能。本研究从太平洋白色对虾中鉴定出一种含有单一C4型Znf结构域（SZnf）的新蛋白，并进一步研究其在免疫中的作用。SZnF蛋白的ZnF结构域与富士塔拉津因子1（FTZ-F1）蛋白的ZnF结构域具有高度同源性，而其它区域与FTZ-F1蛋白的ZnF结构域没有高度同源性。SZnF蛋白主要定位于细胞质中，在细胞核中也有少量表达。SZnF在健康虾的柄和肌肉组织中高表达，在细菌感染期间，其在鳃和肝胰脏中的表达强烈上调。SZnf基因沉默能显著提高对虾对副溶血性弧菌（Vibrio parahaemolyticus）感染的敏感性，但对白色斑点综合征病毒（WSSV）的敏感性无明显影响，提示SZnf基因可能主要参与抗菌反应。双荧光素酶报告基因检测和实时荧光定量PCR分析均表明，SZnf在体内外均能正向调节多种抗菌肽的表达，这可能是其抗菌作用机制的一部分。总之，目前的研究可以帮助更多地了解含ZnF蛋白的功能和甲壳动物对病原体感染的免疫反应的调节机制。
The Zinc finger domains (ZnFs), which contain finger-like protrusions stabilized by zinc ions and function to bind DNA, RNA, protein and lipid substrates, are ubiquitously present in a large number of proteins. In this study, a novel protein containing a single C4 type Znf domain (SZnf) was identified from Pacific white shrimp,Litopenaeus vannameiand its role in immunity was further investigated. The ZnF domain of SZnF but not other regions shared high homology with those of fushi tarazu-factor 1 (FTZ-F1) proteins. The SZnF protein was mainly localized in the cytoplasm and was also present in the nucleus at a small level. SZnF was high expressed in the scape and muscle tissues of healthy shrimp and its expression in gill and heptopancreas was strongly up-regulated during bacterial infection. Silencing of SZnfin vivocould strongly increase the susceptibility of shrimp to infection with Vibrio parahaemolyticus but not white spot syndrome virus (WSSV), suggesting that SZnf could be mainly involved in antibacterial responses. Both dual luciferase reporter assays and real-time PCR analysis demonstrated that SZnf could positively regulate the expression of various antimicrobial peptidesin vitroandin vivo, which could be part of the mechanism underlying its antibacterial effects. In summary, the current study could help learn more about the function of ZnF-containing proteins and the regulatory mechanisms of immune responses against pathogen infection in crustaceans.