The nucleoporin NUP160 andNUP96 regulate nucleocytoplasmic export ofmRNAs andparticipate inethylene signaling andresponse inArabidopsis
The nucleoporin NUP160 andNUP96 regulate nucleocytoplasmic export ofmRNAs andparticipate inethylene signaling andresponse inArabidopsis
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拟南芥中核孔蛋白 NUP160 和 NUP96 调节 mRNA 的核胞质输出并参与乙烯信号传导和反应
DOI：
10.1007/s00299-022-02976-6
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发表时间：
2022
影响因子：
6.2
通讯作者：
Chun‑Hai Dong
中科院分区：
文献类型：
--
作者：
Yuanyuan Nie;Yang Li;Menghui Liu;Binran Ma;Xinying Sui;Jiacai Chen;Yanchong Yu;Chun‑Hai Dong
mRNA在细胞核和细胞质之间的双向运输由核孔复合体控制。（NPC）。在高等植物中，NPC含有至少30个核孔蛋白。拟南芥核孔蛋白参与了多种生物学过程，如病原体相互作用、结瘤、冷反应、开花和激素信号传导。然而，核孔蛋白NUP 160和NUP 96在乙烯信号通路中的调控功能尚不清楚。在本研究中，我们提供的数据表明拟南芥核孔蛋白NUP 160和NUP 96参与乙烯信号相关mRNA的核质转运。拟南芥核孔蛋白突变体（nup 160、nup 96 -1、nup 96-.2）表现出增强的乙烯敏感性。核qRT-PCR分析和poly（A）-mRNA原位杂交表明，核孔蛋白突变体影响所有检测的mRNA的核质转运，包括乙烯信号相关的mRNA，如ETR 2、ERS 1、ERS 2、EIN 4、CTR 1、EIN 2和EIN 3。核孔蛋白NUP 160和NUP 96突变体的转录组分析提供了线索，提示核孔蛋白NUP 160和NUP 96可能通过多种分子机制参与乙烯信号传导。这些结果大大促进了我们对核孔蛋白在乙烯信号和乙烯反应中的调节机制的理解。
The two-way transport of mRNAs between the nucleus and cytoplasm are controlled by the nuclear pore complex.(NPC). In higher plants, the NPC contains at least 30 nucleoporins. The Arabidopsis nucleoporins are involved in various.biological processes such as pathogen interaction, nodulation, cold response, flowering, and hormone signaling. However,.little is known about the regulatory functions of the nucleoporin NUP160 and NUP96 in ethylene signaling pathway. In.the present study, we provided data showing that the Arabidopsis nucleoporin NUP160 and NUP96 participate in ethylene.signaling-related mRNAs nucleocytoplasmic transport. The Arabidopsis nucleoporin mutants (nup160, nup96-1, nup96-.2) exhibited enhanced ethylene sensitivity. Nuclear qRT-PCR analysis and poly(A)-mRNA insitu hybridization showed.that the nucleoporin mutants affected the nucleocytoplasmic transport of all the examined mRNAs, including the ethylene.signaling-related mRNAs such as ETR2, ERS1, ERS2, EIN4, CTR1, EIN2, and EIN3. Transcriptome analysis of the nucleo-.porin mutants provided clues suggesting that the nucleoporin NUP160 and NUP96 may participate in ethylene signaling via.various molecular mechanisms. These observations significantly advance our understanding of the regulatory mechanisms.of nucleoporin proteins in ethylene signaling and ethylene response.