Synergistic effect of white-rot fungi and alkaline pretreatments for improving enzymatic hydrolysis of poplar wood
Synergistic effect of white-rot fungi and alkaline pretreatments for improving enzymatic hydrolysis of poplar wood
复制标题
DOI：
10.1016/j.indcrop.2016.03.041
复制
发表时间：
2016-08
影响因子：
5.9
通讯作者：
Liming Zhang;Tingting You;Tian Zhou;Lu Zhang;Feng Xu
中科院分区：
文献类型：
--
作者：
Liming Zhang;Tingting You;Tian Zhou;Lu Zhang;Feng Xu
白腐菌预处理是一种环境友好的预处理方法，具有化学品和能源消耗少的优点。然而，除了耗时之外，白腐真菌处理导致低效的脱木质素和胡敏素形成，这两者都抑制了随后的酶水解。结合碱处理残余木质素的部分解聚和胡敏素的溶解是白腐菌预处理样品转化的有效选择。在所提出的工作中，组合处理将酶消化率提高到89.3%，相比之下，未处理样品的酶消化率为10.6%，单独白腐处理样品的酶消化率为12.9%。在120 °C下进行8周的白腐处理和碱处理后，纤维素的消化率达到最高，而在160 °C下单独进行碱处理的糖产量最高。有效的转化可以归因于白腐和碱处理之间的协同作用，导致纤维素酶的可及性增加。因此，白腐菌处理是一种绿色工艺，可以有效地降低能耗，并且与碱处理组合在促进糖转化方面是高效的。
White-rot fungi pretreatment is one of the most environment-friendly pretreatment methods because of low chemical and energy requirement. However, apart from time consuming, white-rot fungi treatment results in inefficient delignification and humin formation, both of which inhibit subsequent enzymatic hydrolysis. Combined alkaline treatment for partial depolymerization of residual lignin and dissolution of humin is an efficient option for the conversion of white-rot pretreated samples. In the work presented, combined treatments increased the enzymatic digestibility to 89.3%, compared with 10.6% for untreated sample and 12.9% for solely white-rot treated samples. Highest digestibility of cellulose was achieved after 8 weeks of white-rot treatment and alkaline treatment at 120 °C, whereas sugar yield was highest from sole alkaline treatment at 160 °C. The efficient conversion can be ascribed to synergistic interaction between white-rot and alkaline treatments, resulting in increased enzymatic accessibility of cellulose. Therefore, white-rot fungi treatment is a green process that can efficiently decrease energy consumption, and in combination with alkaline treatment is highly effective in promoting sugar conversion.