Studies on the interaction between Cd2+ ions and nucleobases and nucleotides
Studies on the interaction between Cd2+ ions and nucleobases and nucleotides
复制标题
DOI：
10.1016/s0162-0134(02)00411-7
复制
发表时间：
2002-06-07
影响因子：
3.9
通讯作者：
Huq, F
中科院分区：
文献类型：
--
作者：
Hossain, Z;Huq, F
镉是啮齿类动物的一种强致癌物，最近已被国际癌症研究机构接受为一类（人类）致癌物，但其作用的分子机制仍不清楚。然而，有人认为镉诱导的致癌作用可能涉及Cd2+与DNA的直接或间接相互作用。本研究发现，当Cd2+与腺嘌呤和鸟嘌呤相互作用时，腺嘌呤的高效液相色谱（HPLC）保留时间有明显变化，而鸟嘌呤的保留时间无明显变化。由于Cd2+被认为是与腺嘌呤和鸟嘌呤共价结合的，所以保留时间的变化而在峰值部分中没有镉的存在表明：(i)镉-核碱基加合物的不稳定性，以及（ii）由于共价结合，在腺嘌呤中引入了某种化学修饰，而在鸟嘌呤中没有。这一结果与Ni2+不同，在Ni2+中，鸟嘌呤的滞留时间发生了变化，而腺嘌呤的滞留时间没有变化。(C) 2002爱思唯尔科学有限公司版权所有。
Cadmium is a potent carcinogen in rodents and has recently been accepted by the International Agency for Research on Cancer as a category I (human) carcinogen, but the molecular mechanism of its action remains largely unclear. It has however been suggested that cadmium-induced carcinogenesis may involve either direct or indirect interaction of Cd2+ with DNA. In this study it is found that when Cd2+ is allowed to interact with adenine and guanine, there is a marked change in the high performance liquid chromatography (HPLC) retention time for adenine but not for guanine. Since Cd2+ is believed to bind covalently to adenine and guanine, the changes in retention time but absence of any cadmium in the peak fraction point to the following: (i) lability of cadmium-nucleobase adducts, and (ii) introduction of some kind of chemical modification in adenine but not in guanine as a result of covalent binding. This result is different from that for Ni2+ in which case a change in retention time was observed for guanine but not for adenine. (C) 2002 Elsevier Science Inc. All rights reserved.