Solvent dependence of the fluorescence lifetimes of xanthene dyes
Solvent dependence of the fluorescence lifetimes of xanthene dyes
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DOI：
10.1111/j.1751-1097.1999.tb08277.x
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发表时间：
1999-11-01
影响因子：
3.3
通讯作者：
Seybold, PG
中科院分区：
文献类型：
--
作者：
Magde, D;Rojas, GE;Seybold, PG
在 H2O、D2O 和一系列溶液中测量了五种代表性呫吨染料——罗丹明 B 两性离子 (RB+/-)、罗丹明 B 阳离子 (RB+)、罗丹明 6G 阳离子 (R6G(+))、罗丹明 101 两性离子 (R101(+/-)) 和荧光素双阴离子 (F2-) 的荧光寿命。 在从甲醇到辛醇的醇类溶剂中，随着溶剂从水到辛醇的变化，RB+/-和RB+的寿命显着增加。相比之下，R6G(+)和R101(+/-)的寿命在醇系列中略有下降，而F2-的寿命在同一系列中仅略有增加。对于所有研究的染料，在 D2O 中观察到的荧光寿命比在 H2O 中的荧光寿命稍长。讨论了观察到的变化的可能原因。
Fluorescence lifetimes of five representative xanthene dye species-the rhodamine B zwitterion (RB+/-), the rhodamine B cation (RB+), the rhodamine 6G cation (R6G(+)), the rhodamine 101 zwitterion (R101(+/-)) and the fluorescein dianion (F2-)-were measured in H2O, D2O and in a series of alcohol solvents ranging from methanol to octanol, The lifetimes of both RB+/- and RB+ increased markedly as the solvent was varied from water to octanol, In contrast, the lifetimes of R6G(+) and R101(+/-) decreased slightly over the alcohol series and that of F2- increased only slightly in the same series. For all the dyes studied the fluorescence lifetimes observed in D2O were slightly longer than those in H2O. Possible causes for the variations observed are discussed.