Polymerisation and surface modification of methacrylate monoliths in polyimide channels and polyimide coated capillaries using 660 nm light emitting diodes.
Polymerisation and surface modification of methacrylate monoliths in polyimide channels and polyimide coated capillaries using 660 nm light emitting diodes.
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使用 660 nm 发光二极管对聚酰亚胺通道和聚酰亚胺涂层毛细管中的甲基丙烯酸酯整体进行聚合和表面改性。
DOI：
10.1016/j.chroma.2011.03.021
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Macka,Mirek
中科院分区：
文献类型：
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作者：
Walsh,Zarah;Levkin,PavelA;Abele,Silvija;Scarmagnani,Silvia;Heger,Dominik;Klan,Petr;Diamond,Dermot;Paull,Brett;Svec,Frantisek;Macka,Mirek
报道了以菁染料敏化剂/三苯基丁基硼酸盐/N-甲氧基-4-苯基吡啶四氟硼酸盐为引发体系，在660 nm发光二极管（LED）激发下制备整体分离介质的研究。这项工作证明了多种用途的红光引发的聚合反应中制备的单片固定相内聚酰亚胺和聚酰亚胺涂层通道和单片材料的分子，强烈吸收在紫外线区域的修改。该引发剂复合物用于合成聚（甲基丙烯酸丁酯-共-二甲基丙烯酸乙烯酯）和聚（甲基丙烯酸甲酯-共-二甲基丙烯酸乙烯酯）单片固定相在聚酰亚胺涂层的熔融石英毛细管的不同内径，以及在聚酰亚胺微流控芯片。用一批100 μm i.d.的聚（甲基丙烯酸丁酯-共-二甲基丙烯酸乙烯酯）整料证明了制备过程和所得整料结构的可重复性。聚酰亚胺涂层的熔融石英毛细管，适用于分离的模型蛋白质混合物（核糖核酸酶A，细胞色素C，肌红蛋白和卵清蛋白）。取每个批次的12个色谱图的平均值，蛋白质分离的保留因子（k）的最大相对标准偏差记录为0.53%，选择性因子（α）的最大方差为0.40%，而峰分离度的最大相对标准偏差为8.72%。所有的最大值被记录为核糖核酸酶A/细胞色素C peaks.Scanning电子显微镜证实成功的实验中，使用相同的引发方法，在毛细管和微流控芯片，分别制备聚（甲基丙烯酸丁酯-共-二甲基丙烯酸乙烯酯）整料。该引发体系也被应用到光引发的接枝发色单体到聚（甲基丙烯酸丁酯-共-二甲基丙烯酸乙烯酯）整料内聚（甲基丙烯酸丁酯）涂层的熔融石英毛细管。
An investigation into the preparation of monolithic separation media utilising a cyanine dye sensitiser/triphenylbutylborate/N-methoxy-4-phenylpyridinium tetrafluoroborate initiating system activated by 660 nm light emitting diodes is reported. The work demonstrates multiple uses of red-light initiated polymerisation in the preparation of monolithic stationary phases within polyimide and polyimide coated channels and the modification of monolithic materials with molecules which absorb strongly in the UV region. This initiator complex was used to synthesise poly(butyl methacrylate-co-ethylene dimethacrylate) and poly(methyl methacrylate-co-ethylene dimethacrylate) monolithic stationary phases in polyimide coated fused silica capillaries of varying internal diameters, as well as within polyimide micro-fluidic chips. The repeatability of the preparation procedure and resultant monolithic structure was demonstrated with a batch of poly(butyl methacrylate-co-ethylene dimethacrylate) monoliths in 100 μm i.d. polyimide coated fused silica capillary, which were applied to the separation of a model protein mixture (ribonuclease A, cytochrome C, myoglobin and ovalbumin). Taking an average from 12 chromatograms originating from each batch, the maximum relative standard deviation of the retention factor (k) for the protein separations was recorded as 0.53%, the maximum variance for the selectivity factor (α) was 0.40% while the maximum relative standard deviation in peak resolution was 8.72%. All maxima were recorded for the Ribonuclease A/Cytochrome C peaks.Scanning electron microscopy confirmed the success of experiments in which poly(butyl methacrylate-co-ethylene dimethacrylate) monoliths were prepared using the same initiation approach in capillary and micro-fluidic chips, respectively. The initiating system was also applied to the photo-initiated grafting of a chromophoric monomer onto poly(butyl methacrylate-co-ethylene dimethacrylate) monoliths within poly(tetrafluoroethylene) coated fused silica capillaries.