Chronic estrogen treatment increases levels of endothelial nitric oxide synthase protein in rat cerebral microvessels
Chronic estrogen treatment increases levels of endothelial nitric oxide synthase protein in rat cerebral microvessels
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DOI：
10.1161/01.str.30.10.2186
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发表时间：
1999-10-01
期刊：
影响因子：
8.3
通讯作者：
Krause, DN
中科院分区：
文献类型：
--
作者：
McNeill, AM;Kim, N;Krause, DN
背景和目的——大量研究表明，女性性腺激素、雌激素可以预防中风等脑血管疾病。雌激素产生这些作用的一个假设机制是对内皮一氧化氮合酶 (eNOS) 的作用，该酶产生血管舒张分子 NO。我们研究了雌激素增加雄性和雌性大鼠脑微血管中 eNOS 表达的假设。方法 - 我们通过蛋白质印迹法测量了从 7 组动物中分离出的脑微血管中 eNOS 蛋白的水平：雌性、去势雌性、用雌激素处理的去势雌性、雄性、去势雄性、用雌激素加热的去势雄性和用雌激素加热的去势雄性。结果-用雌激素治疗的去势雌性大鼠脑微血管中 eNOS 蛋白水平比去势雌性高 17.4 倍，完整雌性的 eNOS 蛋白水平比去势雌性高 16.6 倍（P
Background and Purpose-A number of studies indicate that the female gonadal hormone, estrogen, confers protection against cerebrovascular disorders such as stroke. One postulated mechanism for these effects of estrogen is an action on the enzyme endothelial nitric oxide synthase (eNOS), which produces the vasodilatory molecule NO. We have investigated the hypothesis that estrogen increases expression of eNOS in cerebral microvessels of male and female rats.Methods-We measured levels of eNOS protein by Western blot in cerebral microvessels isolated from 7 groups of animals: females, ovariectomized females, ovariectomized females treated with estrogen, males, castrated males, castrated males heated with estrogen, and castrated males treated with testosterone.Results-Ovariectomized female rats treated with estrogen bad 17.4-fold greater levels of eNOS protein in cerebral microvessels than ovariectomized females, and intact females had 16.6-fold greater levels than ovariectomized Females (P