Recent Development of Photo-Cross-Linkers as Tools for Biomedical Research.
Recent Development of Photo-Cross-Linkers as Tools for Biomedical Research.
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DOI：
10.2533/chimia.2018.758
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发表时间：
2018-11
期刊：
影响因子：
1.2
通讯作者：
Yulin Tian;Qing Lin
中科院分区：
文献类型：
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作者：
Yulin Tian;Qing Lin
在药物化学和化学生物学中，光交联剂是识别药物靶点和非靶点以及探测结合部位的宝贵工具。本文综述了基于配体和基因编码的光交联剂的研究进展及其在生物系统中的应用。重点介绍了我们发现的新型光交联剂2-芳基-5-羧基四氮唑(ACT)及其在药物靶标识别和哺乳动物细胞瞬时蛋白质相互作用复合体识别中的成功应用。
Photo-cross-linkers are invaluable tools for identifying drug targets and off-targets as well as probing the binding sites in medicinal chemistry and chemical biology. In this review, we summarize recent development of the ligand-based and genetically encoded photo-cross-linkers and their use in biological system. In particular, we highlight our discovery of 2-aryl-5-carboxytetrazole (ACT) as a novel class of photo-cross-linkers and their successful applications in drug target identification as well as identifying transient protein-protein interaction complexes in mammalian cells.