Brattleboro rat hypothalamic neurons transcribe vasopressin gene: evidence from in situ hybridization.
Brattleboro rat hypothalamic neurons transcribe vasopressin gene: evidence from in situ hybridization.
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布拉特尔伯勒大鼠下丘脑神经元转录加压素基因：来自原位杂交的证据。
DOI：
10.1159/000124669
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发表时间：
1986
影响因子：
4.1
通讯作者：
Pfaff,DW
中科院分区：
文献类型：
--
作者：
McCabe,JT;Morrell,JI;Ivell,R;Schmale,H;Richter,D;Pfaff,DW
原位杂交已用于鉴定正常大鼠和布拉特尔伯勒大鼠中含有加压素 (VP) 或催产素 (OT) mRNA 的特定下丘脑大细胞神经元。与 OT 或 VP mRNA 特异性探针杂交后，下丘脑大细胞核中已识别神经元的亚核分布与含有 VP 或 OT 激素的细胞分布的免疫细胞化学描述直接一致。每个细胞的颗粒数量表明，Brattleboro 大鼠含有更高水平的 OT mRNA，而与正常大鼠相比，与 VP 探针杂交时，Brattleboro 大鼠组织中产生的颗粒较少。本文首次对布拉特尔伯勒大鼠中 VP 基因表达进行了逐个细胞描述，并证明 VP 基因转录精确地局限于正常大鼠中通常合成 VP 激素的大细胞神经元。
In situ hybridization has been used to identify specific hypothalamic magnocellular neurons, in normal and Brattleboro rats, that contain vasopressin (VP) or oxytocin (OT) mRNA. The subnuclear distribution of identified neurons in hypothalamic magnocellular nuclei after hybridization with several probes specific for OT or VP mRNA was in direct agreement with immunocytochemical descriptions of the distribution of cells containing VP or OT hormone. The number of grains per cell suggested that Brattleboro rats contained greater levels of OT mRNA, while hybridization with VP probes produced fewer grains in tissue from Brattleboro rats compared to normal rats. This paper provides the first cell-by-cell description of VP gene expression in the Brattleboro rat and demonstrates that VP gene transcription is confined precisely to the magnocellular neurons that normally synthesize VP hormone in normal rats.