COMPARISON OF INDIRECT IMMUNOFLUORESCENT-ANTIBODY ASSAY, ENZYME-LINKED IMMUNOSORBENT-ASSAY, AND WESTERN IMMUNOBLOT FOR THE DIAGNOSIS OF LYME-DISEASE IN DOGS
COMPARISON OF INDIRECT IMMUNOFLUORESCENT-ANTIBODY ASSAY, ENZYME-LINKED IMMUNOSORBENT-ASSAY, AND WESTERN IMMUNOBLOT FOR THE DIAGNOSIS OF LYME-DISEASE IN DOGS
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DOI：
10.1128/jcm.28.1.92-96.1990
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发表时间：
1990-01-01
影响因子：
9.4
通讯作者：
ONDERDONK, A
中科院分区：
文献类型：
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作者：
LINDENMAYER, J;WEBER, M;ONDERDONK, A
采用酶联免疫吸附试验(EL ISA)、间接免疫荧光抗体试验(IFA)和免疫印迹试验对128份犬血清标本进行伯氏疏螺旋体抗体检测。血清包括72份来自怀疑患有莱姆病的狗的样本，32份来自居住在莱姆病非流行地区的狗的样本，以及24份来自具有免疫介导性疾病(n=10)、落基山斑点热(n=5)或钩端螺旋体病(n=9)的临床和血清学证据的狗的样本。以Western免疫印迹结果为标准，评价两种方法的效价。虽然两种检测方法的特异度相同(93.5%)，但EL ISA的敏感性(84.8%)明显高于IFA(66.7%)。免疫印迹试验阳性的8份标本，经酶联免疫吸附试验和间接免疫荧光法均为阴性。在这八只狗中，四只来自怀疑患有免疫介导性疾病的狗，两只来自怀疑患有钩端螺旋体病的狗，两只来自怀疑患有莱姆病的狗。这些结果可能表明，免疫介导性疾病的狗的血清和钩端螺旋体病的狗的血清与伯氏杆菌抗原发生交叉反应。或者，免疫印迹结果可能不能真实地反映莱姆病的状态，特别是在患有免疫介导性疾病的狗的情况下。然而，目前，使用西方免疫印迹作为狗的诊断标准，提供了对疾病的临床定义的最佳选择。
Enzyme-linked immunosorbent assay (ELISA), indirect immmunofluorescent-antibody assay (IFA), and Western immunoblot were used to test serum samples from 128 dogs for the presence of antibody to Borrelia burgdorferi. Sera included 72 samples from dogs suspected of having Lyme disease, 32 samples from dogs residing in areas in which Lyme disease was not considered endemic, and 24 samples from dogs with clinical and serologic evidence of immune-mediated disease (n = 10), Rocky Mountain spotted fever (n = 5), or leptospirosis (n = 9). Results of Western immunoblotting were used as the standard against which performances of ELISA and IFA were measured. ELISA was significantly more sensitive than IFA (84.8 versus 66.7%), although both tests were equally specific (93.5%). Eight samples that were positive by Western immunoblot were simultaneously negative by ELISA and IFA. OF these eight, four were from dogs suspected of having immune-mediated disease, two were from dogs suspected of having leptospirosis, and two were from dogs suspected of having Lyme disease. These results may indicate that sera from dogs with immune-mediated disease, and to a lesser extent sera from those with leptospirosis, cross-react with B. burgdorferi antigens. Alternatively, Western immunoblot results may not truly reflect Lyme disease status, particularly in the case of dogs with immune-mediated diseases. At present, however, the use of Western immunoblotting as a diagnostic standard for dogs offers the best alternative to a clinical definition of disease.