Long-term effects of resveratrol supplementation on suppression of atherogenic lesion formation and cholesterol synthesis in apo E-deficient mice
Long-term effects of resveratrol supplementation on suppression of atherogenic lesion formation and cholesterol synthesis in apo E-deficient mice
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DOI：
10.1016/j.bbrc.2008.06.113
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发表时间：
2008-09-12
影响因子：
3.1
通讯作者：
Choi, Myung-Sook
中科院分区：
文献类型：
--
作者：
Do, Gyeong-Min;Kwon, Eun-Young;Choi, Myung-Sook
动脉粥样硬化是一种慢性炎症性动脉疾病，由脂质、单核细胞和动脉壁细胞之间的相互作用引起。白藜芦醇补充剂(RV，0.02%和0.06%，w/w)对正常饮食的载脂蛋白E缺陷(apo E-/-)小鼠的调脂、胆固醇合成和抗动脉粥样硬化作用进行了研究。在整个实验期间，白藜芦醇补充组的血浆总胆固醇(TOTAL-C)和低密度脂蛋白-胆固醇(LDL-C)浓度显著低于对照组。CF组和RV组血浆高密度脂蛋白胆固醇浓度显著升高，高密度脂蛋白胆固醇/总胆固醇比值显著高于对照组。0.06%白藜芦醇组血浆对氧磷酶(PON)活性明显高于对照组。氯贝特组和白藜芦醇组大鼠肝脏HMG-CoA还原酶(HMGR)活性明显低于对照组。补充白藜芦醇可减轻载脂蛋白E-/-小鼠动脉粥样硬化病变和动脉周围脂肪沉积。添加白藜芦醇的载脂蛋白E-/-小鼠动脉粥样硬化血管内细胞间黏附分子-1(ICAM-1)和血管细胞黏附分子-1(VCAM-1)的表达减少。这些结果为白藜芦醇在喂养正常饮食的载脂蛋白E-/-小鼠中的抗动脉粥样硬化和降胆固醇特性提供了新的见解。(C)2008 Elsevier Inc.保留所有权利。
Atherosclerosis is a chronic inflammatory disease of the arteries resulting from interactions between lipids, monocytes, and arterial wall cells. The effects of resveratrol supplements (RV, 0.02% and 0.06% each, w/w) with regard to the modulation of lipid profiles, cholesterol synthesis, and anti-atherogenesis were examined in apo E-deficient (apo E-/-) mice fed a normal diet. The concentration of total-cholesterol (total-C) and LDL-cholesterol (LDL-C) in plasma was significantly lower in the resveratrol-supplemented groups compare to the control group over the entire experimental period. The plasma HDL-C concentration was significantly elevated, and the ratio of HDL-C/total-C was significantly higher in the CF and RV groups than in the control group. Plasma paraoxonase (PON) activity was significantly higher in the 0.06% resveratrol group. The hepatic HMG-CoA reductase (HMGR) activity was significantly lower in the clofibrate and resveratrol groups than in the control group. Resveratrol supplements attenuated the presence of atherosclerotic lesions and periarterial fat deposition in the apo E-/- mice. The presence of intracellular adhesion molecule-1 (ICAM-1) and Vascular cell adhesion molecule-1 (VCAM-1) in atherosclerotic vessels was diminished in the resveratrol-supplemented apo E-/- mice. These results provide new insight into the anti-atherogenic and hypocholesterolemic properties of resveratrol in apo E-/- mice that were fed a normal diet. (C) 2008 Elsevier Inc. All rights reserved.