Inhibition of transcriptional regulator Yin-Yang-1 by association with c-Myc.
Inhibition of transcriptional regulator Yin-Yang-1 by association with c-Myc.
复制标题
DOI：
10.1126/science.8266081
复制
发表时间：
1993-12
期刊：
影响因子：
56.9
通讯作者：
Ajay Shrivastava;S. Saleque;G. Kalpana;Steven E. Artandi;Stephen Goff;Kathryn Calame
中科院分区：
文献类型：
--
作者：
Ajay Shrivastava;S. Saleque;G. Kalpana;Steven E. Artandi;Stephen Goff;Kathryn Calame
阴阳一号(YY1)调控多种基因的转录，包括癌基因c-fos和c-myc。根据上下文的不同，YY1可以作为转录抑制因子、转录激活因子或转录启动子。利用酵母双杂交系统筛选与YY1相关蛋白的人互补DNA文库，获得c-myc基因。亲和层析证实YY1与c-Myc结合，但不与Max结合。在共转染中，c-Myc同时抑制YY1的抑制和激活功能，这表明c-Myc的作用方式之一是通过调节YY1的活性。
Yin-Yang-1 (YY1) regulates the transcription of many genes, including the oncogenes c-fos and c-myc. Depending on the context, YY1 acts as a transcriptional repressor, a transcriptional activator, or a transcriptional initiator. The yeast two-hybrid system was used to screen a human complementary DNA (cDNA) library for proteins that associate with YY1, and a c-myc cDNA was isolated. Affinity chromatography confirmed that YY1 associates with c-Myc but not with Max. In cotransfections, c-Myc inhibits both the repressor and the activator functions of YY1, which suggests that one way c-Myc acts is by modulating the activity of YY1.