Transcriptomic profiles of the smoke tree wilt fungus Verticillium dahliae under nutrient starvation stresses
Transcriptomic profiles of the smoke tree wilt fungus Verticillium dahliae under nutrient starvation stresses
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营养饥饿胁迫下烟树枯萎真菌大丽黄萎病的转录组谱
DOI：
10.1007/s00438-015-1052-4
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发表时间：
2015-05
影响因子：
3.1
通讯作者：
Tian Chengming
中科院分区：
文献类型：
--
作者：
Xiong Dianguang;Wang Yonglin;Tian Chengming
大丽轮枝菌是一种广为人知的植物病原菌，在200多种植物上引起维管束枯萎。在植物侵染过程中，病原菌与寄主相互作用过程中的有效营养是成功侵染的必要条件。然而，很少有人关注这种真菌的营养吸收和饥饿反应。在这里，我们使用RNA-Seq来分析大丽弧菌对营养饥饿的反应，包括碳和氮的耗竭。基因表达谱分析表明，在碳饥饿和氮饥饿条件下，有1854个基因差异表达，其中852个上调，539个下调；氮饥饿，487个上调，291个下调。在差异表达基因中，糖酵解、脂肪酸生物合成或代谢、黑色素生物合成等涉及乙酰辅酶A利用或产生的基因在碳饥饿条件下受到抑制，而黑色素生物合成基因在氮饥饿条件下受到强烈诱导。这些结果结合VDH1的表达数据表明，氮饥饿诱导了黑色素的生物合成和微菌核的发育，而碳饥饿抑制了微菌核的发育。此外，许多编码碳水化合物活性酶和分泌蛋白的基因在碳饥饿下被诱导。总体而言，这些结果提高了我们对大丽弧菌对营养饥饿的反应的理解，并有助于识别潜在的毒力因子，以开发新的疾病控制策略。
Verticillium dahliae is a notorious plant pathogen that causes vascular wilt on more than 200 plant species. During plant infection, efficient pathogen nutrition during the interaction with the host is a requisite for successful infection. However, little attention has been focused on nutrient uptake and starvation responses in this fungus. Here, we used RNA-Seq to analyze the response of V. dahliae to nutrient starvation, including carbon and nitrogen depletion. Gene expression profile analysis showed that 1854 genes were differentially expressed under carbon starvation (852 upregulated and 539 downregulated genes) and nitrogen starvation (487 upregulated and 291 downregulated genes). Among the differentially expressed genes, genes involved in utilization or production acetyl-CoA, including glycolysis, fatty acid biosynthesis or metabolism, and melanin biosynthesis, were repressed under carbon starvation, whereas melanin biosynthesis genes were strongly induced under nitrogen starvation. These results, combined with VDH1 expression data, suggested that melanin biosynthesis and microsclerotia development were induced under nitrogen starvation, but microsclerotia development was suppressed under carbon starvation. Furthermore, many genes encoding carbohydrate-active enzymes and secreted proteins were induced under carbon starvation. Overall, the results improve our understanding of the response of V. dahliae to nutrient starvation and help to identify potential virulence factors for the development of novel disease control strategies.
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影响因子：
5.8
作者：
Tong, Mark Y.;Cassa, Christopher A.;Kohane, Isaac S.
通讯作者：
Kohane, Isaac S.
DOI：
10.1016/j.fgb.2014.04.006
发表时间：
2014-11
期刊：
Fungal genetics and biology : FG & B
影响因子：
--
作者：
van Munster JM;Daly P;Delmas S;Pullan ST;Blythe MJ;Malla S;Kokolski M;Noltorp ECM;Wennberg K;Fetherston R;Beniston R;Yu X;Dupree P;Archer DB
通讯作者：
Archer DB
影响因子：
4.5
作者：
Fernandez J;Wright JD;Hartline D;Quispe CF;Madayiputhiya N;Wilson RA
通讯作者：
Wilson RA
影响因子：
12.9
作者：
Sabire Özcan;M. Johnston
通讯作者：
Sabire Özcan;M. Johnston
影响因子：
2.5
作者：
B. Krems;C. Charizanis;K. Entian
通讯作者：
B. Krems;C. Charizanis;K. Entian