High-throughput microfluidic particle velocimetry using optical time-stretch microscopy
High-throughput microfluidic particle velocimetry using optical time-stretch microscopy
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DOI：
10.1063/1.5101015
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发表时间：
2019-07
影响因子：
4
通讯作者：
Yingchun Ding;Liqi Yu;Chaomin Zhang;Huimei He;Bin Zhang;Qiang Liu;Yu Duli;Xing Xiaoxing
中科院分区：
文献类型：
--
作者：
Yingchun Ding;Liqi Yu;Chaomin Zhang;Huimei He;Bin Zhang;Qiang Liu;Yu Duli;Xing Xiaoxing
我们报告了利用光流控时间拉伸显微镜以高通量测量超高速流动的微观粒子的速度。这是一项使用光学时间拉伸显微镜作为粒子测速工具的研究，我们开发了一种定制算法来处理从光流控平台获取的图像，以计算单个粒子的速度。我们通过实验确定了不同尺寸的聚苯乙烯微球以 ~10 000 个颗粒/秒的吞吐量穿过微通道的实际流速。我们还检查了用于粒子测速的具有不同纵横比（深度与宽度）的微流体通道。结果表明可测量流速高达 2.51 m/s。我们的方法为高速量级的无标记和高通量粒子测速提供了一种有前途的工具。我们报告了利用光流控时间拉伸显微镜在高通量下对超高速流动的微观粒子进行速度测量。这是一项使用光学时间拉伸显微镜作为粒子测速工具的研究，我们开发了一种定制算法来处理从光流控平台获取的图像，以计算单个粒子的速度。我们通过实验确定了不同尺寸的聚苯乙烯微球以 ~10 000 个颗粒/秒的吞吐量穿过微通道的实际流速。我们还检查了用于粒子测速的具有不同纵横比（深度与宽度）的微流体通道。结果表明可测量流速高达 2.51 m/s。我们的方法为高速量级的无标记和高通量粒子测速提供了一种有前景的工具。
We report the velocity measurement of microscopic particles flowing at ultrahigh speed with optofluidic time-stretch microscopy at high throughput. This is a study of using optical time-stretch microcopy as a tool for particle velocimetry, where we developed a custom algorithm to process the images acquired from the optofluidic platform for the velocity calculation of individual particles. We experimentally determined the actual flow velocities for polystyrene microspheres with different sizes and traveling through the microchannel at a throughput of ∼10 000 particles/s. We also examined microfluidic channels with different aspect ratios (depth-to-width) for particle velocimetry. The result indicates a measurable flow velocity up to 2.51 m/s. Our method provides a promising tool for label-free and high-throughput particle velocimetry at high velocity magnitudes.We report the velocity measurement of microscopic particles flowing at ultrahigh speed with optofluidic time-stretch microscopy at high throughput. This is a study of using optical time-stretch microcopy as a tool for particle velocimetry, where we developed a custom algorithm to process the images acquired from the optofluidic platform for the velocity calculation of individual particles. We experimentally determined the actual flow velocities for polystyrene microspheres with different sizes and traveling through the microchannel at a throughput of ∼10 000 particles/s. We also examined microfluidic channels with different aspect ratios (depth-to-width) for particle velocimetry. The result indicates a measurable flow velocity up to 2.51 m/s. Our method provides a promising tool for label-free and high-throughput particle velocimetry at high velocity magnitudes.