Differential regulation of the expression of period2 protein in the limbic forebrain and dorsomedial hypothalamus by daily limited access to highly palatable food in food-deprived and free-fed rats
Differential regulation of the expression of period2 protein in the limbic forebrain and dorsomedial hypothalamus by daily limited access to highly palatable food in food-deprived and free-fed rats
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DOI：
10.1016/j.neuroscience.2007.04.044
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发表时间：
2007-06-29
期刊：
影响因子：
3.3
通讯作者：
Amir, S.
中科院分区：
文献类型：
--
作者：
Verwey, M.;Khoja, Z.;Amir, S.
生物钟基因在大脑和身体的许多区域有节奏地表达，并且被认为是大多数内源性昼夜节律行为和生理过程的基础。整个大脑和身体的时钟基因表达的每日节律通常由视交叉上核（SCN）协调，但它们也受到食物供应的每日时间限制的强烈影响。在这里，我们研究了在食物缺乏的患者中，每天限制性地提供高度可口的完全代餐，巧克力Ensure Plus（Ensure）的影响。（限制进食，RF）和自由进食（限制性治疗，RT）大鼠，在涉及动机和情绪调节的大脑区域中时钟蛋白Period 2（PER 2）的表达;这些包括终纹床核卵圆核（BNSTov）、杏仁核中央核（CEA）、基底外侧杏仁核（BLA）、齿状回（DG）和下丘脑背内侧核（DMH）。RF和RT大鼠消耗了相似量的Ensure，但仅在RF条件下观察到PER 2表达模式的变化，这表明这些区域中PER 2表达的变化是由与RF相关的负代谢状态的每日缓解触发的，并且独立于消耗物质本身的正激励特性。与此相反，立即早期基因，Fos的表达，增加了在这些地区的RF和RT时间表，表明有关的刺激价值的消费的食物到达这些地区的信号。在RF或RT大鼠的SCN中未观察到PER 2或Fos表达的变化。这些研究结果表明，机制导致PER 2的表达变化和那些影响RF和RT下Fos的诱导，至少部分是分离的。(c)2007年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Circadian clock genes are rhythmically expressed in many areas of the brain and body and are thought to underlie most endogenous circadian behaviors and physiological processes. Daily rhythms of clock gene expression throughout the brain and body are normally coordinated by the suprachiasmatic nucleus (SCN), but they are also strongly influenced by daily temporal restrictions of food availability. Here, we studied the effects of a daily restricted presentation of highly palatable complete meal replacement, chocolate Ensure Plus (Ensure) in food-deprived (restricted feeding, RF) and free-fed (restricted treat, RT) rats, on the expression of the clock protein, Period2 (PER2) in regions of the brain involved in motivational and emotional regulation; these include the oval nucleus of the bed nucleus of the stria terminalis (BNSTov), the central nucleus of the amygdala (CEA), the basolateral amygdala (BLA), the dentate gyrus (DG) and the dorsomedial hypothalamus (DMH). RF and RT rats consumed similar amounts of Ensure, but changes in the pattern of PER2 expression were seen only in the RF condition, suggesting that changes in PER2 expression in these regions are triggered by the daily alleviation of a negative metabolic state associated with RF and are independent of the positive incentive properties of the consumed substance, per se. In contrast, the expression of the immediate early gene, Fos, was increased in these regions by both RF and RT schedules, showing that signals concerning the incentive value of the consumed food reach these regions. No changes in either PER2 or Fos expression were observed in the SCN of RF or RT rats. These findings demonstrate that mechanisms leading to changes in the expression of PER2 and those affecting the induction of Fos under RF and RT are, at least in part, dissociable. (c) 2007 IBRO. Published by Elsevier Ltd. All rights reserved.