Repetitive fluctuations in blood glucose enhance monocyte adhesion to the endothelium of rat thoracic aorta
Repetitive fluctuations in blood glucose enhance monocyte adhesion to the endothelium of rat thoracic aorta
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DOI：
10.1161/01.atv.0000239488.05069.03
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发表时间：
2006-10-01
影响因子：
8.7
通讯作者：
Watada, Hirotaka
中科院分区：
文献类型：
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作者：
Azuma, Kosuke;Kawamori, Ryuzo;Watada, Hirotaka
背景-本研究的目的是阐明血糖浓度反复波动对单核细胞粘附主动脉内皮的影响。方法和结果-非肥胖型2型糖尿病大鼠Goto-Kakizaki （GK）每天喂食两次，以诱导重复餐后血糖峰值。然后，我们比较了这些大鼠与自由喂养大鼠胸主动脉内皮上粘附的单核细胞数量。为了抑制葡萄糖峰值，研究人员连续12周在每次饭前给大鼠注射一种钠-葡萄糖转运蛋白抑制剂——连根碱。GK大鼠每天喂养两次，其HbA（1c）明显低于自由喂养的GK大鼠。然而，前者的内皮细胞粘附数量明显高于后者，动脉内膜增厚明显增加。根瘤素显著减少GK大鼠每天两次的粘附单核细胞数量。结论-我们的数据表明，餐后葡萄糖浓度的反复波动引起单核细胞粘附内皮细胞，比体内稳定高血糖诱导的更严重。抑制这种波动有效地抑制了单核细胞对主动脉内皮的粘附。
Background - The aim of this study was to elucidate the effect of repetitive fluctuations in blood glucose concentrations on monocyte adhesion to the aortic endothelium.Methods and Results - Nonobese type 2 diabetes, Goto-Kakizaki (GK) rats were fed twice daily to induce repetitive postprandial glucose spikes. Then, we compared the number of monocytes adherent to the endothelium of thoracic aorta in these rats with that in rats fed ad libitum. To suppress the glucose spikes, rats were injected with an inhibitor of sodium - glucose transporter, phloridzin, just before each meal for 12 weeks. GK rats fed twice daily showed significantly lower HbA(1c) than GK rats fed ad libitum. However, the former group showed markedly higher number of monocytes adherent to the endothelium than the latter, together with increased arterial intimal thickening. Phloridzin significantly reduced the number of adherent monocytes in GK rats fed twice daily.Conclusion - Our data demonstrated that repetitive postprandial fluctuation in glucose concentration evokes monocyte adhesion to endothelial cells that was worse than that induced by stable hyperglycemia in vivo. Suppression of such fluctuations efficiently suppressed monocyte adhesion to the aortic endothelium.