Oligosaccharides expressed an MUC1 produced by pancreatic and colon tumor cell lines
Oligosaccharides expressed an MUC1 produced by pancreatic and colon tumor cell lines
复制标题
DOI：
10.1074/jbc.272.39.24198
复制
发表时间：
1997-09-26
影响因子：
4.8
通讯作者：
Hollingsworth, MA
中科院分区：
文献类型：
--
作者：
Burdick, MD;Harris, A;Hollingsworth, MA
MUC 1表达于乳腺、胰腺、气道和胃肠道等组织的导管上皮的顶端表面，其功能包括润滑和保护上皮。此外，MUC 1在肿瘤细胞的粘附和抗粘附特性中的作用已被提出，并且MUC 1串联重复结构域的广泛O-糖基化可能有助于这些功能。关于MUC 1的特异性O-糖基化的信息很少。鉴定不同MUC 1糖型的一个问题是产生的抗MUC 1核心蛋白的单克隆抗体识别串联重复结构域中的表位，所述串联重复结构域通常被糖基化到使这些表位模糊的程度，我们开发了一种表位标记的MUC 1形式，可以检测多种MUC 1糖型，并确定了许多重要的血型和肿瘤的存在。两种胰腺肿瘤细胞系（Panc-1和S2-013）和两种结肠肿瘤细胞系（Caco-2和HT-29）表达的MUC 1上的相关碳水化合物抗原。检测到的抗原包括唾液酸-路易斯（a）、唾液酸-路易斯（c）、唾液酸-路易斯（x）和唾液酸-Tn。
MUC1 is expressed at the apical surface of ductal epithelia of tissues, including breast, pancreas, airway, and the gastrointestinal tract, where its functions include lubrication and protection of the epithelia, In addition, roles for MUC1 have been suggested in both adhesive and antiadhesive properties of tumor cells, and extensive O-glycosylation of the MUC1 tandem repeat domain may contribute to these functions. Little information is available on the specific O-glycosylation of MUC1. One problem in identifying different MUC1 glycoforms has been that monoclonal antibodies raised against the MUC1 core protein recognize epitopes in the tandem repeat domain, which is often glycosylated to an extent that obscures these epitopes, We developed an epitope-tagged form of MUC1 that allowed the detection of multiple MUC1 glycoforms and established the presence of a number of important blood group and tumor-associated carbohydrate antigens on MUC1 expressed by two pancreatic tumor cell lines (Panc-1 and S2-013) and two colon tumor cell Lines (Caco-2 and HT-29). Antigens detected include sialyl-Lewis(a), sialyl-Lewis(c), sialyl-Lewis(x), and sialyl-Tn.