Detection of oncogene mutation from neoplastic colonic cells exfoliated in feces
Detection of oncogene mutation from neoplastic colonic cells exfoliated in feces
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DOI：
10.1007/bf02055116
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发表时间：
1996-11-01
影响因子：
3.9
通讯作者：
Crucitti, F
中科院分区：
文献类型：
--
作者：
Ratto, C;Flamini, G;Crucitti, F
目的：在结直肠癌转移扩散之前获得治愈的最佳机会。缺乏能够对肿瘤进行早期诊断的特定测试，这就解释了通过非侵入性方法调查与致癌有关的基因变化的原因。在本研究中，研究了K-ras密码子12和13在从肠道脱落到粪便中的肿瘤细胞以及肿瘤和正常粘膜中的突变。此外，健康的患者和其他几个癌症前期疾病的患者只是接受了检查。方法：收集25例结直肠腺癌患者的粪便、肿瘤和粘膜标本。11例健康患者的粪便和粘膜标本，3例腺瘤(1例)和溃疡性结肠炎(2例)患者的粪便、病理肠组织和正常粘膜标本。聚合酶链式反应扩增和限制性内切酶分析。结果：10例癌症患者的肿瘤和粪便标本中均检测到K-ras密码子12突变，其中14例未发现基因突变。在一名患有肿瘤的患者中，只在肿瘤组织中显示了突变。肿瘤和粪便分析的符合率为96%。肠粘膜中K-ras密码子12和13的模式正常。在K-ras试验中，健康患者的粪便和粘膜标本未见改变。取自癌前病变患者的样本之间登记了一致意见。结论：这些初步发现表明，在研究排入粪便的结直肠细胞中的K-ras改变方面具有很高的准确率，这表明这种方法可以用于研究其他基因改变，并具有前瞻性地识别早期结直肠癌。
PURPOSE: Best chances of a cure from colorectal cancer are obtained before metastatic spread. Lack of specific tests allowing early diagnosis of the tumor accounts for investigation of gene alterations involved in carcinogenesis by a noninvasive method. in the present study, K-ras codons 12 and 13 mutations were studied in neoplastic cells shed from the bowel into the stool and those contained in the tumor and normal mucosa. Moreover, healthy patients and a few others with precancerous conditions mere examined. METHODS: Stool, tumor, and mucosa samples were taken from 25 patients with colorectal adenocarcinoma. Stool and mucosa samples were obtained from 11 healthy patients, and stool, pathologic bowel tissue, and normal mucosa samples were obtained from 3 patients with adenoma (1) or ulcerative colitis (2). Polymerase chain reaction amplification and restriction enzyme analysis were performed. RESULTS: K-ras codon 12 mutations were detected in both tumor and stool samples of 10 cancer patients, and no gene alterations were observed in 14 patients. In one patient with a tumor, a mutation was shown in only the tumor tissue. The agreement rate in tumor and stool analysis was 96 percent. A normal pattern of K-ras codons 12 and 13 was observed in the bowel mucosa. Ail stool and mucosa samples from healthy patients were not altered in K-ras. Agreement was registered between samples taken from patients with preneoplastic lesions. CONCLUSIONS: These preliminary findings show a high rate of accuracy in the investigation of K-ras alterations in the colorectal cells shed into the feces, suggesting that such an approach could be used to study other gene alterations and, prospectively, to identify early colorectal cancers.