Cytoskeleton and Chromosome Damage Leading to Abnormal Mitosis Were Involved in Multinucleated Cells Induced by Silicon Nanoparticles
Cytoskeleton and Chromosome Damage Leading to Abnormal Mitosis Were Involved in Multinucleated Cells Induced by Silicon Nanoparticles
复制标题
硅纳米颗粒诱导的多核细胞涉及导致有丝分裂异常的细胞骨架和染色体损伤
DOI：
10.1002/ppsc.201400180
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发表时间：
2015-06-01
影响因子：
2.7
通讯作者：
Sun, Zhiwei
中科院分区：
文献类型：
--
作者：
Li, Yang;Jing, Li;Sun, Zhiwei
近年来，非晶硅纳米颗粒（SNPs）被广泛应用于各个领域，特别是生物和医学领域。因此，这些纳米颗粒的不良影响应仔细研究。snp的多核效应在我们之前的研究中首次报道，但其相关机制尚不清楚。因此，本研究的目的是探讨多核细胞形成的机制。两种尺寸的非晶SNPs （Nano‐Si64和Nano‐Si46）被仔细地表征。首先用两个snp处理人肝L‐02细胞24 h，测定细胞毒性和多核细胞率，然后分别研究细胞融合和异常有丝分裂两种形成多核细胞的方式。结果表明，snp在L - 02细胞中产生剂量依赖性和大小相关性的多核效应。异常有丝分裂而非细胞融合是snp引起多核细胞形成的主要原因。这两个snp都可以通过额外的ROS和Ca2+影响细胞骨架的数量和分布，导致有丝分裂和细胞分裂异常。此外，染色体损伤导致相应的G2/M细胞周期阻滞应该是另一个方面，这最终导致L‐02细胞系中多核细胞的形成。
Recently, amorphous silicon nanoparticles (SNPs) are widely used in a variety of fields, especially in biological and medical science. Thus, the adverse effect of these nanoparticles should be carefully investigated. The multinucleation effect of SNPs was firstly reported in our previous studies, while the relative mechanism is still unclear. Therefore, the purpose of this study is to investigate the mechanisms with regard to the formation of multinucleated cells. Two sizes of amorphous SNPs (Nano‐Si64 and Nano‐Si46) are carefully characterized. Cytotoxicity and rate of multinucleated cells are firstly determined after human hepatic L‐02 cells are treated with two SNPs for 24 h. Then cell fusion and abnormal mitosis, two ways could form multinucleated cells, are investigated, respectively. Results indicated that SNPs produce a dose‐dependent and size‐related multinucleation effect in L‐02 cells. Abnormal mitosis instead of cell fusion is the main reason for the formation of multinucleated cells caused by SNPs. Both two SNPs could affect the quantity and distribution of cytoskeleton through extra ROS and Ca2+ leading to abnormal mitosis and cytokinesis. Additionally, chromosome damage resulting in corresponding G2/M cell cycle arrest should be another aspect, which finally leads to the formation of multinucleated cells in L‐02 cell line.