HERPES-SIMPLEX VIRUS TYPE-1 GLYCOPROTEIN-D GENE - NUCLEOTIDE-SEQUENCE AND EXPRESSION IN ESCHERICHIA-COLI
HERPES-SIMPLEX VIRUS TYPE-1 GLYCOPROTEIN-D GENE - NUCLEOTIDE-SEQUENCE AND EXPRESSION IN ESCHERICHIA-COLI
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DOI：
10.1126/science.6289440
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发表时间：
1982-01-01
期刊：
影响因子：
56.9
通讯作者：
ENQUIST, LW
中科院分区：
文献类型：
--
作者：
WATSON, RJ;WEIS, JH;ENQUIST, LW
定位了单纯疱疹病毒1型糖蛋白D （gD）基因的蛋白编码区，并测定了其核苷酸序列。预测的gD多肽氨基酸序列包含许多与其他病毒糖蛋白相同的特征。将该蛋白编码区插入细菌表达质粒中，可在大肠杆菌中合成免疫反应性gd相关多肽。讨论了该系统制备普通型单纯疱疹病毒疫苗的潜力。
The protein coding region of the herpes simplex virus type-1 glycoprotein D (gD) gene was mapped, and the nucleotide sequence was determined. The predicted amino acid sequence of the gD polypeptide contained a number of features in common with other virus glycoproteins. Insertion of this protein coding region into a bacterial expressor plasmid enabled synthesis in E. coli of an immunoreactive gD-related polypeptide. The potential of this system for preparation of a type-common herpes simplex virus vaccine is discussed.