Genomic organization and expression of hamster UDP-N-acetylglucosamine:dolichyl phosphate N-acetylglucosaminyl phosphoryl transferase.
Genomic organization and expression of hamster UDP-N-acetylglucosamine:dolichyl phosphate N-acetylglucosaminyl phosphoryl transferase.
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仓鼠 UDP-N-乙酰氨基葡萄糖：多乙酰磷酸 N-乙酰氨基葡萄糖磷酰基转移酶的基因组组织和表达。
DOI：
10.1093/glycob/5.1.129
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发表时间：
1995
期刊：
影响因子：
4.3
通讯作者：
Krag,SS
中科院分区：
文献类型：
--
作者：
Scocca,JR;Zou,J;Krag,SS
仓鼠尿苷二磷酸N-乙酰-D-葡糖胺：长链磷酸N-乙酰葡糖胺基磷酸转移酶（L-G1 PT）基因长度超过6.5kb，含有9个外显子。外显子大小为63 ~ 214 bp，编码408个氨基酸。内含子长度在85 ~ 1.4kb之间。上游5′端序列包括两个可能的TATA盒、一个可能的CCAAT盒和至少两个可能的GC盒。异源表达在粟酒裂殖酵母中是成功的，并且导致了衣霉素抗性的细胞，并且具有比野生型细胞高12倍的L-G1 PT活性。针对L-G1 PT蛋白的亲水性肽（残基300-320）制备的抗血清与来自中国仓鼠卵巢（CHO）细胞和具有增加的L-G1 PT活性水平的粟酒裂殖酵母细胞的膜样品中的35-36 kDa蛋白反应。在这两种情况下，抗原肽与抗血清检测到的35-36 kDa蛋白质竞争。
The hamster gene for uridine diphosphateN-acetyl-D-glucosamine: dolichyl phosphateN-acetylglucosaminyl phosphoryl transferase (L-G1PT) was found to extend over 6.5 kb and to contain nine exons. The exons ranged in size from 63 to 214 bp, encoding the 408 amino acid protein. The introns ranged from 85 bp to 1.4 kb. Upstream 5′ sequences included two possible TATA boxes, one possible CCAAT box and at least two potential GC boxes. Heterologous expression was successful inSchizosaccharomyces pombe, and resulted in cells that were tunicamycin resistant and had 12-fold more L-G1PT activity than wild-type cells. Antiserum prepared to a hydrophilic peptide (residues 300–320) of the L-G1PT protein reacted with a 35–36 kDa protein in membrane samples from Chinese hamster ovary (CHO) cells andS.pombecells that had increased levels of L-G1PT activity. In both cases, antigenic peptide competed with the 35–36 kDa protein detected by the antiserum.