In Situ Visualization and Quantification of Three-Dimensional Root System Architecture and Growth Using X-Ray Computed Tomography
In Situ Visualization and Quantification of Three-Dimensional Root System Architecture and Growth Using X-Ray Computed Tomography
复制标题
DOI：
10.2136/vzj2014.03.0024
复制
发表时间：
2014-08-01
影响因子：
2.8
通讯作者：
Vetterlein, Doris
中科院分区：
文献类型：
--
作者：
Koebernick, Nicolai;Weller, Ulrich;Vetterlein, Doris
根系结构和相关的根-土相互作用随时间变化很大。为了提高我们对自然土壤根系活动的认识，需要一种非破坏性的方法来量化根系及其时间发育。x射线计算机断层扫描（x射线CT）用于观察和量化蚕豆根系在干燥期间的生长情况。这种植物在受控条件下生长在沙土混合物中，每隔一天进行一次成像。利用闵可夫斯基泛函和欧几里得距离变换对根结构特征进行量化。我们能够对含水量从29.6%下降到6.75%的根系进行成像。与破坏性测量相比，根长略被低估。基于长期的重复测量，有可能量化根系生长的动态和根系沿着土壤深度的人口统计。测量从土壤中任何一点到最近根表面的欧几里得距离，得出水和养分流向根的传播距离的频率分布。我们的研究结果表明，根系及其时间动态的有意义的定量表征是可能的。
Root system architecture and associated root-soil interactions exhibit large changes over time. Nondestructive methods for the quantification of root systems and their temporal development are needed to improve our understanding of root activity in natural soils. X-ray computed tomography (X-ray CT) was used to visualize and quantify growth of a single Vicia faba L. root system during a drying period. The plant was grown under controlled conditions in a sandy soil mixture and imaged every second day. Minkowski functionals and Euclidean distance transform were used to quantify root architectural traits. We were able to image the root system with water content decreasing from 29.6 to 6.75%. Root length was slightly underestimated compared with destructive measurements. Based on repeated measurements over time it was possible to quantify the dynamics of root growth and the demography of roots along soil depth. Measurement of Euclidean distances from any point within the soil to the nearest root surface yielded a frequency distribution of travel distances for water and nutrients towards roots. Our results demonstrate that a meaningful quantitative characterization of root systems and their temporal dynamics is possible.