QUANTITATIVE-ANALYSIS OF BIOFILM THICKNESS VARIABILITY
QUANTITATIVE-ANALYSIS OF BIOFILM THICKNESS VARIABILITY
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DOI：
10.1002/bit.260450607
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发表时间：
1995-03-20
影响因子：
3.8
通讯作者：
DALY, D
中科院分区：
文献类型：
--
作者：
MURGA, R;STEWART, PS;DALY, D
对铜绿假单胞菌、肺炎克雷伯菌以及这两个物种的二元种群组合的生物膜的厚度变异性进行了量化。实验方法包括用商业组织包埋剂冷冻包埋生物膜，切片，并应用图像分析来构建沿沿着线性横断面（长度达1 cm）的厚度分布。用这种方法包埋和切片的生物膜局部薄如单个细胞附着在表面（
The thickness variability of biofilms of Pseudomanas aeruginosa, Klebsiella pneumoniae, and the binary population combination of these two species was quantified. The experimental method involved cryoembedding biofilms with a commercial tissue embedding agent, sectioning, and applying image analysis to construct thickness profiles along linear transects (up to 1 cm in length) across the substratum. Biofilms embedded and sectioned by this method were locally as thin as a single cell attached to the surface (