Recent advances in the nucleic acid-based diagnostic tool for coronavirus.

Recent advances in the nucleic acid-based diagnostic tool for coronavirus.
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DOI:
10.1007/s11033-020-05889-3
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发表时间:
2020-11
影响因子:
2.8
通讯作者:
Lakhanpal J
Lakhanpal J
中科院分区:
生物学4区
文献类型:
--
作者:
Prabhakar PK;Lakhanpal J

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最近,中国爆发了一种新型冠状病毒,引发了类似肺炎的症状。这种冠状病毒属于SARS和MERS家族,会导致被称为COVID-19的呼吸系统疾病。目前我们采用基于聚合酶链反应(PCR)的分子生物学方法检测冠状病毒。除了这些基于PCR的方法外,也存在一些改进的方法,如基于微阵列的技术,Real - time-quantitative PCR,基于CRISPR-Cas13的工具,但几乎所有可用的方法都有优缺点。这种方法有许多局限性,因此需要一种快速、更灵敏、更有针对性的诊断工具,以便在更短的时间内检测到更多的样品。在这里,我们总结了目前可用的基于核酸的冠状病毒检测诊断方法,以及开发更快、更灵敏的冠状病毒感染检测技术的必要性。基于核酸的SARS-CoV-2检测工具
Recently in China, a novel coronavirus outbreak took place which caused pneumonia-like symptoms. This coronavirus belongs to the family of SARS and MERS and causes respiratory system disease known as COVID-19. At present we use polymerase chain reaction (PCR) based molecular biology methods for the detection of coronavirus. Other than these PCR based methods, some improved methods also exist such as microarray-based techniques, Real time-quantitative PCR, CRISPR-Cas13 based tools but almost all of the available methods have advantages and disadvantages. There are many limitations associated with this method and hence there is a need for a fast, more sensitive, and specific diagnostic tool which can detect a greater number of samples in less time. Here we have summarised currently available nucleic acid-based diagnostic methods for the detection of coronavirus and the need for developing a better technique for a fast and sensitive detection of coronavirus infections. Nucleic acid based detection tool for SARS-CoV-2.
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