Recent advances in the nucleic acid-based diagnostic tool for coronavirus.
Recent advances in the nucleic acid-based diagnostic tool for coronavirus.
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DOI:
10.1007/s11033-020-05889-3
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发表时间:
2020-11
影响因子:
2.8
通讯作者:
Lakhanpal J
中科院分区:
文献类型:
--
作者:
Prabhakar PK;Lakhanpal J
Recently in China, a novel coronavirus outbreak took place which caused pneumonia-like symptoms. This coronavirus belongs to the family of SARS and MERS and causes respiratory system disease known as COVID-19. At present we use polymerase chain reaction (PCR) based molecular biology methods for the detection of coronavirus. Other than these PCR based methods, some improved methods also exist such as microarray-based techniques, Real time-quantitative PCR, CRISPR-Cas13 based tools but almost all of the available methods have advantages and disadvantages. There are many limitations associated with this method and hence there is a need for a fast, more sensitive, and specific diagnostic tool which can detect a greater number of samples in less time. Here we have summarised currently available nucleic acid-based diagnostic methods for the detection of coronavirus and the need for developing a better technique for a fast and sensitive detection of coronavirus infections. Nucleic acid based detection tool for SARS-CoV-2.
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DOI:
10.1038/nrmicro2147
发表时间:
2009-06
期刊:
Nature reviews. Microbiology
影响因子:
--
作者:
通讯作者:
--
影响因子:
7.4
作者:
Cai, Sheng;Jung, Cheulhee;Ellington, Andrew D.
通讯作者:
Ellington, Andrew D.
影响因子:
2.7
作者:
Noh JY;Yoon SW;Kim DJ;Lee MS;Kim JH;Na W;Song D;Jeong DG;Kim HK
通讯作者:
Kim HK
影响因子:
3.3
作者:
Nagamine, K;Hase, T;Notomi, T
通讯作者:
Notomi, T
影响因子:
2.7
作者:
Hadjinicolaou, Andreas V.;Farcas, Gabriella A.;Kostrikis, Leondios G.
通讯作者:
Kostrikis, Leondios G.